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Hanging drop preparation is a, special type of wet mount (in, which a drop of medium, containing the organisms is, placed on a microscope slide),, often is used in dark, illumination to observe the, motility of bacteria., , Depression (well) Cover slip, * Petroleum jelly (seal), ( —* ), , Drop of liquid with specimen, (hanging upside down, from undersurface of cover slip)

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In this method, a drop of, culture is placed on a coverslip, that is encircled with petroleum, jelly (or any other sticky, material). The coverslip and, drop are then inverted over the, well of a depression slide. The, drop hangs from the coverslip,, and the petroleum jelly forms a, seal that prevents evaporation., This preparation gives good, views of microbial motility., , Materials Required

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Glass slides (glass slide with, depression) or normal glass, slide with adhesive or, paraffin ring, , Paraffin wax, , Loop, , Coverslip, , Microscope, , Bunsen burner, , Young broth culture of

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motile bacteria (e.g. Proteus, mirabilis), , Quality Control, , Validate the competence of, technologists in the hangingdrop method by testing known, motile enterococci or Listeria in, the broth assay., , Slide Preparation, , etroleum jell, . aoe drop of bacteria

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ee ee, cover|slip, depression slide, , Hanging Drop Method, Preparation, , ¢ Take a clean glass slide and, apply a paraffin ring,, adhesive-tape ring to make, circular concavity. (This step, is not needed if a glass slide, with depression is available)., , ¢ Hold a clean coverslip by its, edges and carefully dab, vaseline on its corners using, a toothpick.