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Core Paper XI, Molecular Biology, , Unit 1: Nucleic Acids, DNA Replication & Repair, , Salient features of DNA and RNA, Watson and Crick model of DNA.,Nucleic acids cot, curves, denaturation and renaturation of DNA, DNA Replication in prokaryotes and, eukaryotes, mechanism of DNA replication, Semi-conservative, bidirectional and semidiscontinuous replication, RNA priming, Replication of circular and linear ds-DNA,, replication of telomeres, Pyrimidine dimerization and mismatch repair., , Unit 2: Transcription & Translation, RNA polymerase and transcription Unit, mechanism of transcription in prokaryotes and, , eukaryotes, synthesis of rRNA and mRNA, transcription factors 1 Of, transcription. 16 / 54, Genetic code, Degeneracy of the genetic code and Wobble Hypothesi. Aeid, , synthesis in prokaryotes: Ribosome structure and assembly in prokaryotes, tiaenty or protein, synthesis, aminoacyl tRNAsynthetases and charging of tRNA; Proteins involved in initiation,, elongation and termination of polypeptide chain; Inhibitors of protein synthesis; Difference, between prokaryotic and eukaryotic translation., , Unit 3: Post Transcriptional Modifications and Processing of Eukaryotic RNA, Structure of globin mRNA; Split genes: concept of introns and exons, splicing mechanism,, alternative splicing, exon shuffling, and RNA editing, Processing of tRNA., , Unit 4: Gene Regulation & Regulatory RNAs, , Transcription regulation in prokaryotes: Principles of transcriptional regulation with, examples from lac operon and trp operon; Transcription regulation in eukaryotes: Activators,, repressors, enhancers, silencer elements; Gene silencing, RNA interference, miRNA, siRNA., , PRACTICAL, 1. Study of Polytene chromosomes from Chironomous / Drosophila larvae, , 2. Preparation of liquid culture medium (LB) and raise culture of E. coli, , 3. Estimation of the growth kinetics of E. coli by turbidity method, , 4. Preparation of solid culture medium (LB) and growth of E. coli by spreading and streaking, 5. Quantitative estimation of Salmon sperm/calf thymus DNA _ using colorimeter, (Diphenylamine reagent) or spectrophotometer (A2s9nm measurement), , 6. Quantitative estimation of RNA using Orcinol reaction, , 7. Study and interpretation of electron micrographs/ photograph showing, , (a) DNA replication, (b) Transcription and (c) Split genes., , TEXT BOOKS, , 1. Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition., John Wiley and Sons. Inc., , 2. Lewin B. (2013). Gene XI, Jones and Bartlett., , 3. De Robertis E.D.P. (2017) Cell and Molecular Biology 8Ed., , 4. Arnold Berk,Chris A. Kaiser, Harvey Lodish, Angelika Amon, Hidde, Ploegh, Anthony Bretscher, Monty Krieger Kelsey C. Martin(2016) Molecular Cell, Biology. 8" edition., , 16