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nitrocellulose or nylon membrane is referred t, 9. First biochemical to be produced commercially, Pradeep's A TEXT BOOK OF BIOLOGY (XI), IV/70, PRACTICE QQUESTIONS, I., Multiple Choice Questions, 1. Insect resistance transgenic cotton has been, produced by inserting a piece of DNA from, (a) an insect, (c) a wild relative of cotton, (d) a virus, by microbial cloning and genetic engineerine, (b) Penicillin, (d) Fertility factor, (BHU PMT 2005), 10. Golden rice is a transgenic crop of the fut, with the following improved trail :, (a) Human insulin, (c) Interferons, (b) a bacterium, (CPMT 2004), 2. Hybridoma technology has been successfully, used in, (a) Insect resistance, (b) High lysine (essential amino acid) content, (c) High protein content, (d) High vitamin-A content, 11. Bacillus thuringiensis (Bt) strains have been used, for designing novel, (a) Biofertilizers, (b) Bio-metallurgical techniques, (c) Bio-mineralization processes, (d) Bio-insecticidal plants, 12. Which of the following is a correct statement:, (a) "Bt" in "Bt-cotton" indicates that it is a, genetically modified organism produced through, biotechnology, (b) Somatic hybridization involves fusion of two, complete plant cells carrying desired genes, (c) The anticoagulant hirudin is being produced, from transgenic Brassica napus seed, (d) "Flavr Savr" variety of tomato has enhanced, the production of ethylene which improves its taste, (a) production of somatic hybrids, (b) synthesis of monoclonal antibodies, (c) synthesis of haemoglobin, (d) production of alcohol in bulk, (AIPMT 2005), (J & K CET 2004), 3. During "gene cloning" which is called as "gene, taxi" ?, (a) Vaccine, (c) Bacterium, (b) Plasmid, (d) Protozoa, (UGET Manipal 2004), (AIPMT 2005), 4. Name the drug used in cancer treatment, produced by using biotechnology, (a) Terramycin, (c) Insulin, (e) Interferon, 5. Cultivation of Bt cotton has been much in the, news. The prefix Bt means, (a) "Barium treated" cotton seeds, (b) "Bigger thread" variety of cotton with better, tensile strength, (c) Produced by "biotechnology" using restriction, enzymes and ligases, (d) Carrying an endotoxin gene from Bacillus, thuringiensis., 6. The first antibiotic was discovered by..., (a) Louis Pasteur, (c) W. Fleming, (b) HGH, (d) TSH, (Kerala PMT 2004), (AIIMS 2005), 13. Which bacteria is used as biopesticide first of, the commercial scale in the world ?, (a) Bacillus thuringiensis, (b) Е. сoli, (c) Pseudomonas aeruginosa, (d) Agrobacterium tumefaciens. (HP PMT 200, 14. Gene recombinant technology is used for, (a) vectorless gene transfer into target cell, (b) vector based gene transfer into target cen, (c) direct transfer of DNA protein complex, (d) liposome base direct gene transfer into tar, cell, 20, (AIIMS 2004), (b) R. Koch, (d) A. Fleming, 21, (КСЕТ 2004), 7. In transgenics, expression of transgene in target, tissue is determined by, (a) Transgene, (c) Reporter, (b) Promoter, (a), (b), (c), (d) Enhancer, (AIPMT 2004), (HP PMT 2006), 8. Terminator gene, (a) Help in terminating flowering, (b) Help in terminating seed germination, (c) Used in hybridisation, (d) None of these, 15. Transfer of DNA bands from an agrose ge, (d), as, (b) northern transfer, (d) gene transfer, (Kerala PMT 2000, (e), (a) western transfer, (c) eastern transfer, (e) southern transfer, (BHU PMT 2005), ANSWERS, 16., 1. (b), 10. (d), 9. (a), 26., 2. (b), 11. (d), 3. (b), 12. (c), 4. (e), 13. (a), 7. (b), 8. (b), 5. (d), 14. (b), 6. (d), 15. (e)
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BIOTECHNOLOGY AND ITS APPLICATIONS, (a) Bacillus thuringiensis 1. Production of chitinases, (d) Pseudomonas putida 4. Production of Bt toxin, F BIOLOGY, 16. Hirudin is, (a) a protein produced by Hordeum vulgare, which, is rich in lysine, (h) a toxic molecule isolated from Gossypium, hirsutum, which reduces human fertility, (c) a protein produced from transgenic Brassica, napus which prevents blood clotting, (d) an antibiotic produced by a genetically, engineered bacterium Escherichia coli, IV/71, (a) a-2, b-4, c-1, d-5, e-3, (b) a-2, b-4, c-5, d-1, e-3, (c) a-4, b-3, c-5, d-2, e-1, roduced commer, | genetic engineri, Penicillin, (d) a-3, b-4, c-5, d-1, e-2, (e) a-4, b-2, c-5, d-3 e-1, 22. Human insulin is being commercially produced, from a transgenic species of, (Kerala PMT 2008), Fertility factor, (a) Rhizobium, (BHU PMT, nic crop of the f, ved trail:, (b) Saccharomyces, (d) Mycobacterium, (AIIMS 2006), 17. Golden rice is a promising transgenic crop., When released for cultivation, it will help in, (a) producing a petrol-like fuel from rice, (b) alleviation of vitamin A, (c) Escherichia, (AIPMT 2008), 23. A transgenic food crop which may help in solving, the problem of night blindness in developing, countries is, amino acid) comten, (c) pest resistance, (d) heribcide tolerance, 18. A genetically engineered micro-organism used, successfully in bioremediation of oil spills is a, species of, (a) Trichoderma, (c) Bacillus, (a) Bt soybean, (AIPMT 2006), (b) Golden rice, (c) Flavrsavr tomatoes (d) Starlink maize, at, (AIPMT, ) strains have bea, (AIPMT 2008), 24. Main objective of production/use of herbicide, resistant GM crops is to, (b) Хаnthomoпas, aniques, (d) Pseudomonas, (a) Encourage eco-friendly herbicides, (b) Reduce herbicide accumulation in food articles, for health safety, (c) Eliminate weeds from the field without the use, ocesses, (AIPMT 2007), (AIPMT, 19. In cloning of cattle a fertilized egg is taken out, of the mother's womb and, is a correct statemet, " indicates that ita, anism produced the, (a) In the eight cell stage, cells are separated and, cultured until small embryos are formed which are, implanted into the womb of other cows., (b) In the eight cell stage, the individual cells are, separated under electrical field for further, development in culture media., (c) From this upto eight identical twins can be, produced., (d) The egg is divided into 4 pairs of cells which, are implanted into the womb of other cows., of manual labour, (d) Eliminate weeds from the field without the use, of herbicide, n involves fusion d, ying desired genes, irudin is being prá, napus seed, of tomato has enhu, e which improves is, (AIPMT 2008), 25. Genetically engineered bacteria are being, employed for production of, (а) Thyroxine, (b) Human insulin, (c) Cortisol, (d) Epinephrine, (WB JEE 2009), 26. Isolation of Bt gene from bacterium (Bacillus, thuringiensis) was taken up in the year, (AIIMS, (AIPMT 2007), 20. Blindness is prevented by use of which crop in, poor countries ?, (a) Golden rice, I as, biopesticide fi, (a) 1977, (b) 1980, a the world?, (c) 1997, (d) 1990, 27. Which one of the following is commonly used in, transfer of foreign DNA into crop plants ?, (AMU 2009), is, (b) Wheat, (c) Gram, (d) Pea, 21. Match List I with ListI and select the correct, (DPMT 2007), efaciens. (HP PMT, hnology is used for, sfer into target cel, ransfer into target cel, NA protein compler, (HP PMT, inosa, option, (a) Meloidogyne incognita, (b) Agrobacterium tumefaciens, (c) Penicillium expansum, List I, List II, (d) Trichoderma harzianum, (b) Rhizobium meliloti, (e) Escherichia coli, (АIPMТ 2009), 2. Scavenging of oil spills, 3. Incorporation of 'nif", 28. What is true about Bt toxin ?, ds from an agrose, A membrane is refe, ct gene transfer in, (a) Bt toxin exists as active tox, in the Bacillus., gene, (b) The activated toxin enters the ovaries of the, pest to sterilise it and thus prevent its, multiplication, (e) Trichoderma, 5. Production of human, (d) gene transfer, (Kerala PMT, insulin, 16. (c), 26. (b), (b) northerm transer, ANSWERS, 17. (b) 18. (d) 19. (a) 20. (a), 27. (b), 21. (c) 22. (c) 23. (b) 24. (b) 25. (b), 2. (a), 8. (b)
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41. Which of the following Bt crops is being grown, 40. Amplification of gene of interest by using DNA, 36. Silencing of m-RNA has been used in producing, Pradeep's A TEXT BOOK OF BIOLOGY (XI), IV/72, (c) The concerned Bacillus has anti toxins, (d) The inactive prototoxin gets converted into, active form in the insect gut. (AIPMT 2009), transgenic plants resistant to, (a) bollworms, (c) white rusts, (b) nematodes, (d) bacterial blights, (AIPMT Mains 2011), 29. Transgenic plants are the ones, (a) Generated by introducing foreign DNA into a, cell and regenerating a plant from the cell., (b) Produced after protoplast fusion in artificial, medium, 37. The first clinical gene therapy was given for, treating, (a) diabetes mellitus, (c) rheumatoid arthritis, (b) chicken pox, (c) Grown in artificial medium after hybridization, in the field., (d) adenosine deaminase deficiency, (d) Produced by a somatic embryo in artificial, medium, (AIPMT Mains 2012, (AIPMT 2009), 30. The bacterium Bacillus thuringiensis is widely, used in contemporary biology as, (a) Insecticide, 38. The figure below shows three steps (A, B, C)e, Polymerase Chain Reaction (PCR). Select the, option giving correct identification together with, what it represents ?, (b) Agent for production of dairy products, (800 (c) Source of industrial enzyme, Region to be amplified, 5, 3-, 3' ds, -5'DNA, sbbl (d) Indicator of water pollution (AIPMT 2009), 31. Golden rice is :, -3', (a) A variety of rice grown along the yellow river in, China, B, -5', (b) Long stored rice having yellow colour tint, (c) A transgenic rice having gene for B- carotene, (d) Wild variety of rice with yellow coloured grains, (NCERT Exemplar), 5, 151, C, 3 87, (a) B-denaturation at a temperature of about 98°C, adhu separating the two DNA strands, (b) A-denaturation at a temperature of about 50°C, (c) C-extension in the presence of heat stable DNA, polymerase, (d) A-annealing with two sets of primers., 32. In RNAI, genes are silenced using :, 2a (a) ss DNA, -5', (b) ds DNA, (d) ss RNA, (c) ds RNA, (NCERT Exemplar), 33. The first clinical gene therapy was done for the, (800 treatment of:, (a) AIDS, (c) Cystic fibrosis, (d) SCID (Servere Combined Immuno Deficiency, resulting from deficiency of ADA), (b) Cancer, (AIPMT Mains 2012), 39. Tobacco plants resistant to a nematode have been, developed by the introduction of DNA that, produces (in the host cells), (a) both sense and anti-sense RNA, (b) a particular hormone, (c) an antifeedant, (d) a toxic protein., (NCERT Exemplar), 34. ADA is an enzyme which is deficient in a genetic, disorder SCID. What is the full form of ADA ?, (a) Adenosine deoxyaminase, (b) Adenosine deaminase, (AIPMT Mains 2012), (c) Aspartate deaminase, (d) Arginine deaminase, 35. Silencing of a gene could be achieved through, the use of :, (NCERT Exemplar), polymerase may go upto, (a) 0.1 million times, (b) 1.0 million times, (d) 1.0 trillion times., (AMU 2012, (c) 1.0 billion times, (a) short interfering RNA (RNAI), (b) antisense RNA, (c) by both, (d) none of the above, in India by the farmers ?, (NCERT Exemplar), (a) Cotton, (b) Brinjal, ANSWERS, (c) Soybean, (d) Maize, (NEET 2013, 28. (d), 37. (d) 38. (a) 39. (a) 40. (c), 29. (a) 30. (a) 31. (c), 32. (c), 41. (a), 33. (d), 34. (b), 35. (c) 36. (b)
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C. If Assertion is true but the Reason is false., D. If both Assertion and Reason are false., 1. Assertion. Industrial fermentations are money making ventures., B. If both Assertion and Reason are true but Reason is not a correct explanation of the Assertion., 42. The first human hormone produced by, IV/73, bligh, Mains, recombinant DNA technology is, (b) Estrogen, (d) Progesterone, 47. The first clinical gene therapy was given for, treating, (a) diabetes mellitus, (a) Insulin, vas gihe, (c) Thyroxin, (b) chickenpox, (c) rheumatoid arthritis, (AIPMT 2014), 4. An analysis of chromosomal DNA using the, Southern hybridization technique does not use, (d) adenosine deaminase deficiency, pox, (AIIMS 2015), 48. Which kind of therapy was given in 1990 to a, four-year old girl with adenosine deaminase, [ADA] deficiency ?, (a) Gene therapy, (c) Immunotherapy, (a) Electrophoresis, (c) Autoradiography, (b) Blotting, (d) PCR, Main, ps (A. BO, CR). Selet, n together, (b) Chemotherapy, (d) Radiation therapy, (AIPMT 2014), 44. In vitro clonal propagation in plants is, characterized by, (NEET 2016), (a) PCR and RAPD, (c) Electrophoresis and HPLC, (d) Microscopy, 45. An alga which can be employed as food for, human being is, 49. In India, the organisation responsible for, assessing the safety of introducing genetically, modified organisms for public use is, (a) Indian Council of Medical Research (ICMR), (b) Northern blotting, (AIPMT 2014), (b) Council for Scientific and Industrial Research, (CSIR), (c) Research Committee on Genetic Manipulation, (RCGM), (d) Genetic Engineering Appraisal Committee, (GEAC)., (a) Ulothrix, (c) Spirogyra, (b) Chlorella, (d) Palasiphema, (AIPMT 2014), 46. Match the following and select the correct, option:, (a) Earthworm, (NEET 2018), (i) Pioneer species, (ii) Detritivore, (c) Ecosystem service (iii) Natality, (d) Population growth (iv) Pollination, 50. A 'new' variety of rice was patented by a, foreign company, though such varieties have, been present in India for a long time. This is, related to, (b) Succession, ature of abou, rands, (a) Co-667, (c) Lerma Rojo, (b) Sharbati Sonora, (d) Basmati., (a), (a) (), (b) (iv), (c) (iif), (d) (i), (b), (c), (iii), (d), (NEET 2018), 51. Use of bioresources by multinational companies, and organisations without authorisation from, the concerned country and its people is called, ature of abour, (if), (iv), (i), (ii), of heat stable, (iii), (ii), (iv), (i), (iii), (AIPMT 2014), (i), (a) bio-infringement (b) biopiracy, (c) biodegradation, of primers, (iv), IPMT Main, nematode ha, ction of DNA, (d) bioexploitation., (NEET 2018), II., Assertion-Reason Type Questions, RNA, PMT Mains, nterest by, questions consist of two statements each, printed as Assertion and Reason. While answering these, ons you are required to choose any one of the following four responses., P h Assertion and Reason are true and the Reason is a correct explanation of the Assertion., 1.0 trillion i, CAME, 1.0 million ti, A, crops is being F, D., C, b) Brinjal, Maize, B, ANSWERS, 42. (a), 43. (d) 44. (d), 45. (b) 46. (d) 47. (d) 48. (a) 49. (d) 50. (d) 51. (b), 36. ()
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3. Assertion. The antibiotics produced by Streptomyces species have found greatest commercial application., 12. Assertion. Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated, Reason. Both bacteria and yeast multiply very fast to form huge populations which express the desired gene, 14. Assertion. Recognition site should be preferably single and responsive to commonly used restriction enzyme, 1. B; 2. A; 3. C; 4. B; 5. A; 6. D; 7. C; 8. A; 9, C; 10. C; 11. A; 12. D; 13. A; 14. A., Reason. In PBR322 alien DNA is ligated generally in the area of Bam HI site of tetracyline resistance gene, Reason. Some of the important life saving antibiotics such as penicillin and polymixin-B are produced by, Reason. A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop, 13. Assertion. In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes), Pradeep's A TEXT BOOK OF BIOLOGY (XII), 2. Assertion. Bread and other such baked products are porous and soft., Reason. Both CO, and alcohol escape during baking., IV/74, A, D, 4. Assertion. Insulin is an important life saving drug for diabetic patients., Reason. In is now possible to produce insulin by using recombinant DNA technology., Streptomyces., TMIA, 5. Assertion. Monoclonal antibodies are ideal for diagnosis of diseases caused by closely related pathooene, Reason. Monoclonal antibodies are far more specific and reproducible than the antibodies produced bu, conventional techniques., C, A, D, 6. Assertion. Yeasts should not be used in brewing and baking industries., Reason. They produce several harmpul products during brewing and baking., A, 7. Assertion. Cholesterol is one of the most important steroid in animals and human beings., Reason. It is present as a component of animal cell walls and acts as an agent of cell wall extension., (RI, C, ollol, of its, 8. Assertion. The technologies developed in the laboratories have to be gradually scaled up to industrial levels., Reason. To obtain the product in large scale., com, A, prote, Ever, D, The to, B, 9. Assertion. Terrorists are trying to adopt bioweapons these days., Reason. Bioweapons are liked by terrorist organizations as these are costly., ecolo, relatic, given, and "), called, A, C, 10. Assertion. Biopiracy is being resorted to by the countries of North., Reason. Countries of South are incapable of biopiracy., affect t, 11. Assertion. Indians have a "prior art" for antidiabetic property of 'Karela'., Reason. A foreign company has got a patent for antidiabetic property of 'Karela'., individ, physico, morpho, aspects, from the, D, C, A, with the roots of all cereal and pulse crops., (AIIMS 2005, Ecc, increased, physical,, agricultu, with which bacterium is associated., C, yeast (eukaryote), (AIIMS 2005, The, energy su, plant sped, resources, D, A., (AIIMS 2012, ANSWERS
