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Aim:, , be, , taken., , or, , epiaermis, , and a, mond, surface of a dicot, upper and lower, of, safranin. Leat samples, plant material, glycerine,, , microscope,, , Study of distribution of stomata on, , for the, , Requirements : Varnish, distilled water,, Solanum can, Hibiscus, Balsam, Bougainvillea, Petunia, Cassia and, , Theory, , meant, , helps, has, of stomata. Monocot leaf, , aquatic plants., , epidermis, lower, Stomata may be present on upper, have, Stomata: Stomata are pore like openings., cells. Dicotylednous plants, cells known as guard, are surrounded by special type of, Or the leaf. These, dumbel shaped guard cellsS., have, plants, kidney shaped guard cells where as monocotyledonous, the, are, pores having, in transpiration. Stomata, exchange of gases and, stomata on both, , Stomata are, , minutes., , 8., , Observe the slide under microscope., , Mount the peel in glycerine., , 5. Dip the leaf in water., leaf respectively from varnished surface with the help of, 6. Peel off the upper and lower surfaces of, the leaf obliquely., forceps or the leaf peel can be obtained by tearing, peel with safranin. After 2-3 minutes wash the peel so that excess of stain is removed., , upper surface., the lower surface., , surrounded by gurad cells. Gurad cells regulate the opening, surface. Stomata are absent in submerged, Surtaces whereas dicots have more stomata on lower, Procedure:, , 1., , On other leaf coat this thin, , 2., , 3., Allow leaves to, , Select any leaf of the plant to be studied., On one leaf coat a thin layer of varnish on the, layer of vanish on, , 4., , Stain the, , dry for 5-10, , 7., , 9., , 10. Count the number of stomata in both peels., 11. Note down the observed and counted number of stomata in upper and lower surface of leaf., , 12. Perform the same experiment with Monocot and Dicot leaf and note down the count number of stomata.

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Observation:, , Stomatal opening, , Epidermal cell, , -Guard cell, Stomatal, , Chloroplast, , so, , cells, , Stomatal guard, , *****, , epidermis, , Structure of stomata in Monocot leaf, , Number of stomata, , Lower, , that upper surface of leaf is towards, , Upper epidermis, , Structure of stomata in Dicot, , Monocot/Dicot, , Stomatal guard cells, , Open stomata in Dicot Leaf, , stomata are., , Name of plant, , Inference :, S. No., , 1., , 2, 3., , 4, In Monocots,, , In Dicots, stomata are, , Coat the leaf with varnish, , Precautions :, , 1., , before plucking it., peel it off., varnished leaf in water then, , the slide, Mount the peel with varnished surface touching, , 2. Dip the, 3., , objective lens.