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M. Sc. [I], , Zoology, , Savitribai Phule Pune University, (Formerly University of Pune), , Two Year Degree Program in Zoology, (Faculty of Science & Technology), , Revised Syllabi for, M.Sc. (Zoology) Part-I, (for Colleges Affiliated to Savitribai Phule Pune University), , Choice Based Credit System Syllabus, To be implemented from Academic Year 2019-2020, , Savitribai Phule Pune University, , 1

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M. Sc. [I], , Zoology, , Title of the Course: M.Sc. (Zoology), Preamble, Zoology is a major subject of Basic Sciences which deals with all aspects of animal, biology. It includes an interesting range of highly diverse topics. The advancements in, biological Sciences demands, a zoology student to be a master of many areas in the, subject. This Postgraduate degree program has been designed by the Board of Studies in, Zoology of SavitribaiPhule Pune University with a tangible understanding of what is, needed from zoologists and what zoologists need to pursue as a skilled career. It, emulates closely the Benchmark Statement for Biosciences and the guidelines laid down, by the University Grants Commission, New Delhi. This Newly designed Curriculum is an, appropriate blend of the classical aspects in Zoology which has been the “backbone”, knowledge required for all zoologists and the recent and specialized areas. The, flexibility in the Curriculum allows the students to choose their areas of interest leading, to enhanced employability. Students will be provided sufficient number of hours for, their skill development through the Lab Courses and the Project component. The lab, courses have differing flavours and priorities to make a good zoologist. This degree, offers specialization in areas like Genetics, Animal Physiology and Entomology along, with a range of core courses like Biochemistry, Molecular Biology, Comparative Animal, Physiology, Developmental Biology, Environmental Biology etc. Various cross cutting, issues relating to Environmental biology have been aptly included to develop the, students’ sense towards human wellbeing. The field trip/surveys and study tours are, included to gives the student an enticing taste of what life is specially outside the walls, of the classroom. On successful completion of the programme, the students are expected, to understand the key life processes of human and other animal groups, the functioning, of molecules, cells, tissues, organs and systems. Also the students will gain increased, confidence to use initiative and judgement to make decisions in complex and, changeable situations and reflect critically and analytically on personal experience and, make informed decisions about further study, training and employment opportunities., The Master of Science (M.Sc.) in Zoology is a Postgraduate program under the Faculty of, Science and Technology of SavitribaiPhule Pune University Pune. The curriculum designed, Savitribai Phule Pune University, , 2

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M. Sc. [I], , Zoology, , encompasses subjects like Physiology, Entomology, Genetics, Cell Biology, Developmental, Biology,, , Endocrinology,, , Biochemistry,, , Molecular, , Biology,, , Freshwater, , Zoology,, , Environmental Biology etc. Both classical and applied subjects of Zoology have been rightly, blended to offer holistic understanding of the subject., The Choice Based Credit System (CBCS) will be implemented through this curriculum. This, curriculum would certainly felicitate students to develop a strong base of the fundamentals, and specialize in the desired area of their fondness and abilities. The students pursuing this, program would get a privilege to select optional subjects of their choice. A total of 210 hours, for theory lectures and 180 hours for laboratory work have been prescribed in each semester, including a research project to inculcate the research culture amongst students. This newly, designed curriculum will allow students to acquire the skill in handling scientific instruments, planning and performing in the laboratory and exercising critical judgement, independent, thinking and problem solving skills., , Savitribai Phule Pune University, , 3

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M. Sc. [I], , Zoology, , M.Sc. Zoology - Course structure & Distribution of Credits, M.Sc. Zoology, Part –I, Semester-I, Sr., , Core Compulsory, , Choice Based, , Theory/, , No., , Theory Paper, , Optional Paper, , practical, , (CCTP), , (CBOP), , 1, , Credit, , Core Compulsory, , Credit, , Practical Paper, (CCPP), 4, , ZOUT 111, Biochemistry and, Biochemical, Techniques, , 2, , -, , 4, , ZOUT 112, Cell Biology and, Developmental, Biology, , 3, , -, , 4, , ZOUT 113, Genetics andEnglish, in Scientific, Communication., , 4, , -, , -, , ZODT, , Biostatistics/, , 114, , Freshwater, , Theory., , Zoology, , 4, 2, , ---------------------------------, , ZODP, Zoology Practical, , 114, -, , Practical, , Paper-1, , 5, , 2, 4, , ZOUP 115, -, , -, , Basic Zoology Lab-I, Total Credit of Semester 1, , Savitribai Phule Pune University, , 20, , 4

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M. Sc. [I], , Zoology, , M.Sc. Zoology, Part-I, Semester-II, Sr., , Core Compulsory, , Choice Based, , Theory/, , No., , Theory Paper, , Optional, , practical, , (CCTP), , Paper (CBOP), , 1, , Credit, , Core Compulsory, , Credit, , Practical Paper, (CCPP), 4, , ZOUT 121, Molecular Biology, and Bioinformatics, , 2, , -, , 4, , ZOUT 122, Endocrinology and, Parasitology, , 3, , -, , 4, , ZOUT 123, Comparative, Animal Physiology, and, Environmental, Biology., , 4, , -, , -, , ZODT, , Metabolic, , 124, , pathways /, , Theory., , Ichthyology, , ------------ZODP, 124, -, , 4, 2, , Zoology Practical, Paper-2, , 2, , Practical, , -, , 5, , 4, , ZOUP 125, Basic Zoology Lab-, , -, , II, Total Credit of Semester 2, , Savitribai Phule Pune University, , 20, , 5

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M. Sc. [I], , Zoology, , M.Sc. Zoology, Part-II, Semester - III, Sr., , Core Compulsory, , Choice Based, , Theory/, , Credit, , Core, , No., , Theory Paper (CCTP), , Optional Paper practical, , Compulsory, , (CBOP), , Practical, , Credit, , Paper, (CCPP), 1, , ZOUT 231, Special Paper (any one), Animal Physiology-I/, , 4, , Entomology-I/, Genetics-I, 2, , -, , -, , ZOUT 232, Fundamentals of, Systematics, , 4, , and, Economic Zoology, 3, , -, , -, , ZOUT 233, Research Methodology, and, , 4, , Insect Physiology and, Biochemistry, 4, , ZODT, , Immunology/, , 234, , Genetic, , Theory, , Toxicology/, , --------ZODP, 234, -, , -, , Practical, , 5, , 2, 4, , Zoology Practical, Paper-3, , 2, ZOUP 235, , -, , Savitribai Phule Pune University, , -, , 4, , Special Lab I, , 6

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M. Sc. [I], , Zoology, , M.Sc. Zoology, Part II, Semester – IV, Sr., , Core Compulsory, , Choice Based, , Theory/, , No., , Theory Paper (CCTP), , Optional Paper, , practical, , Credit, , Core, , Credit, , Compulsory, , (CBOP), , Practical, Paper, (CCPP), , 1, , ZOUT 241, , 4, , Special Paper-Any OneAnimal Physiology-II/, Entomology-II/, Genetics-II, 2, , -, , -, , ZOUT 242, , 4, , Mammalian, Reproductive, Physiology and, Aquaculture, 3, , ZODT 243 Histology and, Theory., , Histochemistry/, , 4, 2, , Pest Control, ----------ZODP 243, 4, , Zoology, Practical Paper-4, , 2, , Practical, , -, , ZODT 244 Pollution, Theory., , Biology. /, , 4, 2, , Apiculture, -----------ZODP 244, -, , Zoology, Practical Paper-5, , Practical, , 5, , 2, ZOUP 245, , -, , -, , 4, , (Project), Total Credit of Semester 4, , Savitribai Phule Pune University, , 20, , 7

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M. Sc. [I], , Zoology, , Detailed Syllabus with Recommended Books:, Program outcomes (POs): After successfully completing the M.Sc. Zoology, program students will be able to:, PO1. Zoology knowledge: Apply the knowledge of Zoology, Life Sciences and allied, subjects to the understanding of complex life processes and phenomena., PO2. Problem analysis: Identify, review research literature, and analyse complex situations, of living forms., PO3. Design/development of solutions: Design processes/strategies that meet the specified, needs with appropriate consideration for the public health and safety, and the cultural,, societal, and environmental considerations., PO4. Conduct investigations of complex problems: Use research-based knowledge and, research methods including design of experiments, analysis and interpretation of data, and, synthesis of the information to provide valid conclusions in real situations., PO5. Modern tool usage: Create, select, and apply appropriate techniques, resources, and, ICT tools for understanding of the subject., PO6. The Postgraduate and society: Apply reasoning informed by the contextual, knowledge to assess societal, health, safety, legal and cultural issues and the consequent, responsibilities relevant to the professional engineering practice., PO7. Environment and sustainability: Understand the impact of the natural and, anthropogenic activities in societal and environmental contexts, and demonstrate the, knowledge of, and need for sustainable development. Identify a range of invertebrates and, vertebrates and justify their conservation., PO8. Ethics: Apply ethical principles and commit to professional ethics and responsibilities, and norms of the work/research practice., , Savitribai Phule Pune University, , 10

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M. Sc. [I], , Zoology, , PO9. Individual and team work: Function effectively as an individual, and as a member or, leader in diverse teams, and in multidisciplinary settings., PO10. Communication: Communicate effectively on complex life activities with the, scientific community and with society at large, such as, being able to comprehend and write, effective reports and design documentation, make effective presentations, and give and, receive clear instructions., PO11. Project management and finance: Demonstrate knowledge and understanding of, Zoology and management principles and apply these to one’s own work, as a member and, leader in a team., PO12. Life-long learning: Recognize the need for, and have the preparation and ability to, engage in independent and life-long learning in the broadest context of technological change., , Savitribai Phule Pune University, , 11

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M. Sc. [I], , Zoology, , Following is the syllabus of each course along with the course outcomes:, Course Code and Course Name:, ZOUT 111 Biochemistry and Biochemical Techniques., , (4 Credits: 60 Lectures), , Semester I, After successfully completing this course, students will be able to:, CO1: Define basic terms in biochemistry and biochemical techniques., CO2: Explain the applications of the various biochemical techniques., CO3: Explain the structure and functions of various biomolecules., CO4: Explain the importance of tools and techniques in biology., CO5: Illustrate the importance of pH, buffer and water in living systems., CO6: Illustrate the principle, working and applications of basic techniques used in biology., CO7:Draw the structures of various carbohydrates and amino acids., CO8: Classify enzymes with examples., Biochemical techniques:, CO1: Explain the importance and applications of techniques in biochemistry., CO2: Explain theprinciple and applications of various chromatographic techniques with, examples., CO3: Explain the principle, working, materials used and applications of electrophoresis., CO4: Describe the concept of light, electromagnetic spectrum and its application in, absorption spectroscopy., CO5: Illustrate the importance of radioactive compounds and radioactivity in biology., CO6: Demonstrate the principle and working of Warburg’s apparatus., CO7: Demonstrate the principle, working, applications of centrifugation., CO8: Justify the applications of radioactivity compounds in biology., Savitribai Phule Pune University, , 12

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M. Sc. [I], , Zoology, , CO9: Compare the various separation techniques., Sr., , Name of the topic, , Lectures, , No., , allotted, Biochemistry:, , 1., , Basics of chemistry- Structure of atoms, molecules and chemical bonds,, , (02L), , Normality, molarity, molality, 2., , Chemistry of Water : Structure of water and physicochemical properties of, , (04L), , water, water as universal solvent, pH and Buffers, Biological Buffer System, 3., , Carbohydrates: Classification, basic Structures and functions, Biological, , (03L), , Significance., 4., , Lipids: Classification, structure and function and biological significance., , (03L), , 5., , Vitamins: Classification- water-soluble and fat-soluble vitamins, Biological, , (02L), , significance., 6., , Amino acids: Classification, properties (Physical properties- Optical, , (02L), , Isomerization, Absorption in UV light, Ionization; Chemical propertiesReactions with carboxyl group and amino group), 7., , Proteins:, , (05L), , a. Peptide bond formation, Ramachandran plot., b. Protein structure:-Primary structure, Secondary structure, Tertiary structure, and Quaternary structures with examples, Biological significance of proteins., 8., , Enzymes:, , (09L), , a. Classification, nomenclature and properties, b. Enzyme kinetics -one substrate reaction (Michaelis-Menten Equation), c. Factors affecting enzyme activity, d. Enzyme inhibition, e. Allosteric Enzymes., f. Isozymes (LDH) and coenzymes, g. Clinical and industrial significance- Any 3, Biochemical Techniques:, 1., , Chromatography: Principle, working and applications of: Adsorption (8L), chromatography Partition chromatography, Ion-exchange chromatography,, Molecular, , exclusion, , chromatography,, , Affinity, , chromatography,Gas, , chromatography, High Performance Liquid Chromatography., Savitribai Phule Pune University, , 13

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M. Sc. [I], , 2., , Zoology, , Electrophoresis: Moving boundary electrophoresis, zone electrophoresis, (5L), different supports used for electrophoresis, electrophoresis of proteins,, electrophoresis of Nucleic acids, isoelectric focussing., , 3., , Absorption spectroscopy: Concepts of light & electromagnetic spectrum, (5L), Ultraviolet and Visible spectrophotometry, atomic absorption spectrometry and, applications., , 4., , Radioactivity: Properties of radioisotopes, commonly used isotopes, structure & (3L), working of G.M, counter, isotopic dilution analysis, use of isotopes in biology,, radiation hazards., , 6., , Manometry: Respiratory quotient determination, Principle of Warburg’s, , (2L), , apparatus, working and applications., 7., , Methods for protein sequencing., , (4L), , N-terminal sequencing, C-terminal sequencing., Methods for DNA sequencing., Maxam- Gilbert Sequencing, Chain termination method, Dye terminator sequencing, 8., , Centrifugation:, , Principle,, , Types, , of, , centrifugation-, , Preparative, , and (3L), , Analytical.applications of centrifugation., , REFERENCE BOOKS:, 1.Biochemistry, 3rd Ed. (2005), Voet Donald and Voet Judith G. John,, Publisher: Wiley & sons, New York., 2.Biochemistry 6th Ed, (2007) Berg Jeremy, Tymoczko John, StryerLubert,, Publisher: W. H. Freeman, New York., 3.Lehninger’s Principles of Biochemistry, 4th edition, (2005) Nelson D. L. and, Cox M. M. W. H. Freeman & Co. NY., 4.Biochemical Calculations, 2nd Ed., (1997) Segel Irvin H., Publisher: John, Wiley and Sons, New York., 5.Enzymes: Biochemistry, Biotechnology & Clinical chemistry, (2001) Palmer, Trevor, Publisher: Horwood Pub. Co., England., 6.Biochemistry, Geoffrey Zubay, William C Brown Pub; 4th edition (June 1999), Savitribai Phule Pune University, , 14

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M. Sc. [I], , Zoology, , 7.Principles and Techniques of Biochemistry and Molecular Biology, 6th edition, (2008), Keith Wilson and John Walker, Publisher–Cambridge University Press., 8.Light Microscopy in Biology: A Practical Approach, 2nd edition (1999), Alan, J. Lacey, Publisher–Oxford University Press., 9.Electron Microscopy: Principles and Techniques for Biologists, (1992), Lonnie, D. Russell, Publisher-Jones & Bartlett, , Course Code and Course Name:, ZOUT 112 Cell Biology and Developmental Biology(4 Credit: 60 Lecture), Semester I, After successfully completing this course, students will be able to:, Cell Biology:, CO1: Label the various cell parts, CO2: Sketch and label various types of cells and cell organelles., CO3: Explain carbon as backbone of biomolecules., CO4: Explain the ultrastructure and functions of various cell organelles., CO5: Explain the concepts of cell signalling., CO6: Illustrate the chemistry and organization of cytoskeleton., CO7: Illustrate the types, development and causes of tumor., CO8: Diagrammatically represent the cell cycle phases and its regulation., , Developmental Biology:, CO1: Define the terms in developmental biology, CO2: Explain the significance of model organism for developmental studies., CO3: Explain the types of eggs, concept of fertilization and cleavage pattern., CO4: Explain the concept of mesoderm induction and pattern formation with examples., CO5: Describe neural competence and induction., CO6: Explain the concept of growth and differentiation., CO7: Illustrate postembryonic development., CO8: Compare and contrast spermatogenesis and oogenesis., Savitribai Phule Pune University, , 15

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M. Sc. [I], , Sr., , Zoology, , Name of the topic, , Lectures, , No., , allotted, , 1., , Cell theory, general organisation of cell and characteristics of living systems., , (02L), , 2., , Overview of chemical nature of the cell and importance of carbon in biologically, , (02L), , important molecules., 3., , Plasma membrane: Ultrastructure, membrane proteins; membrane transport –, , (04L), , channels, carriers and pumps; membrane potential and synaptic transmission., 4., , Endomembrane system: (Endoplasmic reticulum, Golgi complex, Lysosomes);, , (04L), , protein trafficking – secretory and endocytic pathways., 5., , Mitochondria: Structure, genetic system, functions; protein import, , (03L), , 6., , Nucleus: Ultrastructure – nuclear envelope, nuclear lamina, nuclear pore, , (03L), , complex, nucleolus., 7., , Cell Cycle: Phases, check points, mechanism of regulation; regulators of cell, , (03L), , cycle progression – MPF, cyclins and cyclin dependent kinases (CDKs)., 8., , Cytoskeleton: Types, chemistry, organisation, associated proteins and their role., , (03L), , 9., , Cancer: Types, development, causes; characteristics of cancer cell, tumour, , (03L), , viruses, oncogene and tumour suppresser gene., 10., , Cell signalling: Signalling molecules and their receptors, cell surface (03L), receptors,significance of cell signalling., Developmental Biology:, , 1., , Introduction to developmental biology:, , (3L), , Early theories of Developmental biology Concepts of Developmental biology –, Growth, cell division, cell differentiation, cell, , communication, signalling,, , patterning, induction and competence., 2., , Model Organisms:, , (3L), , Invertebrate: Drosophila melanogaster, Pisces: Zebra Fish- Danio rerio,, Amphibians: Xenopuslaevis, Birds: Chicken, Mammals: Mouse., 3., , Gametogenesis: Spermatogenesis: spermatogenesis, structure of sperm,, , (2L), , regulation of sperm motility, Oogenesis: structure of ovum, previtellogenesis,, vitellogenesis and post-vitellogenesis, 4., , Fertilization:: Concept of fertilization, types of fertilization, Species specific, , (4L), , sperm attraction, recognition of egg & sperm, acrosome reaction, signal, transduction, molecular strategy to avoid polyspermy in fertilization, Savitribai Phule Pune University, , 16

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M. Sc. [I], , 5., , Zoology, , Types of eggs: Based on amount of distribution of yolk, Cleavage: types and, , (2L), , significance., 6., , Morphogenesis: Blastulation: Amphiouxus, Frog, Chick, Mid Blastula, , (5L), , Transition,, Comparative study of Gastrulation in Amphioxus, Frog, Chick, Neurulation: Frog, Chick, Organogenesis: Development of the vertebrate eye –formation of eye field, cell, differentiation, 7., , Pattern formation: Setting up the body axis, , (4L), , 1. Animal vegetal axis: Amphibians,, 2. Dorsal ventral axis: Amphibians, 3. Antero-posterior axis: Drosophila-role of bicoidnanos hunchback., 8., , Organizers:, , (2L), , Spemann and Mangold: Primary embryonic induction, Functions of organizer,, Molecular mechanisms of Amphibian axis formation, 9., , Regeneration:, , (3L), , Limb regeneration: Salamander, Regeneration in Hydra, 10., , Apoptosis, aging and senescence., , (2L), , REFERENCE BOOKS:, 1. Karp Gerald (2010) Cell Biology. 6th Edition, John Willey & Sons (Asia), Pte. Ltd., 2. Cooper Geoffrey M. (1997) The Cell: A Molecular Approach. ASM, Press, Washington D.C., U.S.A., 3. Sadava David E. (1993) Cell Biology – Organelle Structure and, Function. Jones &Barlett Publishers, Boston, London., 4. Hardin Jeff, Gregory Bertoni and Lewis J. Kleinsmith (2012) World of, the Cell. 8th Edition, Pearson Education, Inc., San Francisco, U.S.A., 5. Alberts B., A. Johnson, J. Lewis, M. Raff, K. Roberts and P. Walter, (2008) Molecular Biology of the Cell. 5th Edition, Garland Science, New, York, U.S.A., 6. Lodish H., D. Baltimore, A. Berk, L. Zipursky, M. Matsudaira and J., Darnell (1995) Molecular Cell Biology. Eds. 3, Scientific American &, Savitribai Phule Pune University, , 17

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M. Sc. [I], , Zoology, , W. H. Freeman, New York., 7. De Robertis E. D. P. and De Robertis E. M. E. (1987) Cell and, Molecular Biology. 8th Edition, Lea and Febiger, Philadelphia., 8. Loewy A. G., P. Siekevitz, J. R. Menninger and J. A. N. Gallant (1991), Cell Structure & Function. Saunders College Publishing, U.S.A., 9. Nelson D. L. and Cox M. M. (2008) Lehninger Principles of, Biochemistry. 5th Edition, W. H. Freeman & Co. NY., 10. Developmental Biology, 9th edition (2010), S.F. Gilbert. Publisher –, Sinauer,Associates Inc., 11. Principles of Development, 3rd edition (2007), Lewis Wolpert, PublisherOxford,University Press., 12. An Introduction to Embryology, 5th edition (2004), B. I. Balinsky., Publisher – ThomasAsia Pvt. Ltd., 13. Developmental Biology, (2001), R. M. Twyman, Publisher - Bios, Scientific, Publishers LTD., 14. Analysis of Biological Development , 2000, Klaus Kalthoff, McGrawHill Science/Engineering/Math; 2nd Ed edition., , Course Code and Course Name:, ZOUT 113 Genetics and English in Scientific Communication., , (4 Credit: 60 Lecture), , Semester I, After successfully completing this course, students will be able to:, Genetics:, CO1: Define the basic terminologies in genetics., CO2: Identify genetic disorders based on Karyotypes and traits., CO3: Explain the concept of Mendelian genetics, gene, gene regulation and multiple alleles., CO4: Discuss Linkage and crossing with their types and significance., CO5: Explain the principles of Population genetics., CO6: Illustrate the modified Mendelian laws of inheritance., CO7: Justify the inheritance of qualitative and quantitative traits., , Savitribai Phule Pune University, , 18

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M. Sc. [I], , Zoology, , CO8: Solve the problems based on gene frequency., English in Scientific Communication:, CO1: Write the outline of a scientific paper., CO2: Write the title, abstract, discussion and citations of a given scientific article., CO3: Prepare a scientific presentation using PowerPoint., CO4: Explain language as a tool for effective scientific communication., CO5: Use the formal elements of specific types of scientific writing., CO6: Critically analyze data from research; incorporate it into assigned writing clearly,, concisely, and logically; and attribute the source with proper citation., CO7: Practice the unique qualities of professional rhetoric and writing style, such as sentence, conciseness, clarity, accuracy, honesty, avoiding wordiness or ambiguity, using direct, order organization, readability, coherence and transitional devices., CO8: Justify the importance of plagiarism check and Proof-read given article., Sr., , Name of the topic, , No., 1., , Lectures, allotted, , Recapitulation of Mendelian principles; Practical applications of genetics in (02L), brief, , 2., , Classical concept of a gene:Allele, pseudoalleles, multiple alleles (blood groups), , (02L), , 3., , Gene interactions and epistasis and their types., , (02L), , 4., , Linkage and crossing over: Linkage, linkage groups, types of crossing over, sex, , (06L), , linkage, sex limited and sex influenced characters,, Recombination,recombination maps in diploids for 2 point and 3 point test cross,, (determination of gene order with suitable examples), 5., , Quantitative genetics : Polygenic inheritance, heritability and its, , (03L), , measurementsand Quantitative Traits Locus mapping, 6., , Principles of Population Genetics: Hardy-Weinberg law and its application for, , (05L), , autosomal genes. Calculations of gene frequencies with suitable examples., 7., , Somatic cell genetics and its applications., , (02L), , 8., , Microbial genetics : Methods of genetic transfers – transformation, conjugation,, , (03L), , transduction, Savitribai Phule Pune University, , 19

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M. Sc. [I], , Zoology, , 9., , Extra chromosomal inheritance:Inheritance of Mitochondria., , (01L), , 10., , Human genetics: dominant and recessive disorders, physical traits., , (04L), , English in Scientific Communication:, 1., , Language as a communication tool; relationship among reading, writing, (2L), listening and speaking, , 2., , Pragmatic competence:co-operative principles and politeness principles, , (1L), , 3., , Organisation of English language: sentence structure, basic grammar, sequence, , (3L), , and tenses, syntax, paragraphs, paraphrases and précis writing, synonyms and, antonyms, 4., , Common errors in written and spoken English: tautology, double negative,, , (2L), , double positive, superfluous words, 5., , Oral presentation: How to prepare a presentation, power point slides, use of, , (2L), , communication and IT, voice, speed of delivery, obstacles in effective, communication, 6., , Drafting of a researchproject for financial assistance from funding agency, (2L), writing of scientific paper using word processor., , 7., , Outline of a scientific research paper:, , (14L), , 7.1Hypothesis, theory and concept, 7.2Title designing, framing Abstract and Keywords, 7.3 Introduction: statement of the problem and justification; aim, objectives,, need, significance and rationale of the study, review of literature., 7.4Materials, , and, , Methods:, , contents,, , importance, , of, , measurements,, , reproducibility etc., 7.5Observations and Results: text and data presentation, tables, graphs,, histograms, diagrams, photographic plates, legends and captions, 7.6Discussion: logical sequence and critical analysis of ideas and evidences, data, conclusion, 7.7 References: finding references from journals, books and databases; Citation styles of citations, 7.8Summary, Acknowledgements, 8., , Editing and correcting: proof-reading symbols, jargons and abbreviations, , (3L), , 9., , Plagiarism: meaning, types, avoiding plagiarism., , (1L), , Savitribai Phule Pune University, , 20

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M. Sc. [I], , Zoology, , REFERENCE BOOKS:, 1. Genetics, 3Rd Edn by Strickberger, Pearson India, 2015, Paperback,, 9789332555105, 2. Principles of Genetics,Gardner, E.J., Peter & Simmons, M.J. and, Snustad, D.P. 8thEdn.John Wiley and Sons, New York, 2006., 3. Concepts of Genetics. William S Klug and Michael R Cummings., 10thEdn. Pearson Education India, 2016., 4. Lewin, Benjamin. Genes IX. John Wiley and Sons, New York,2008., 5. Genetics By Verma,PS. And Agrawal, VK., S.Chand and Co.,New Delhi, 6. Genetics By Gupta, PK., Rastogi Publication, Meerut, 7. Genetics By Sarin,C., Tata McGraw Hill,New Delhi, 8. Genetics: Daniel J Fairbanks, W. Ralph Andersen; Brooks / Cole Publ., co. (1999)., 9. iGenetics: A Molecular Approach, 3rdEdn by Peter J Russell, Pearson, India, 2016, Paperback, 9789332571624, 10. O’Conner M. and F. P. Woodford (1975) Writing Scientific Papers in, English. Elsevier-ExcerptaMedica-North Holland Publ., Amsterdam., 11. Trelease S. F. (1958) How to Write Scientific and Technical Papers., Williams and Wilkins Co. Baltimore, U.S.A., 12. Day Robert (1996) How to Write and Publish a Scientific Paper., Cambridge University Press., 13. McMillan V. (1997) Writing Papers in the Biological Sciences. Edn. 2,, W. H. Freeman, New York., 14. Winkler Anthony C. and Jo Ray McCuen-Metherell (2008) Writing the, Research Paper, A Handbook. 7th Edition, Wadsworth Cengage, Learning, Boston, M.A., U.S.A., 15. Vijayalakshmi G. and C. Sivapragasam (2008) Research Methods – Tip, & Techniques. M.J.P. Publishers, Chennai., 16. Kothari C. R. (2009) Research Methodology: Methods & Techniques. 2nd, Revised Edition, New Age International Publishers, New Delhi., 17. Levinson Stephen C. (2003) Pragmatics. Cambridge University Press,, Cambridge., 18. Yule George (2012)Pragmatics (Oxford Introduction to Language Study, Savitribai Phule Pune University, , 21

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M. Sc. [I], , Zoology, , ELT). Oxford University Press., 19. Quirk Randolph and Greenbaum Sidney (2006) A University Grammar, of English. Pearson Education Ltd., 20. Editors of Merriam Webster (2006) Webster’s English Usage Guide., Federal Street Press, Springfield, M. A., U.S.A., 21. Wren P. C. and H. Martin (2016) High School English Grammar and, Composition. Blackie ELT Books (A Division of S. Chand & Co. Pvt., Ltd.), New Delhi, India., 22. American Psychological Association(2010)Publication Manual of the, American Psychological Association. 6th Edition, Washington D.C., 23. Modern Language Association(2016)MLA Handbook. 8th Edition, The, Modern Language Association of America., , Course Code and Course Name:, ZODT 114 Biostatistics (2 Credits= 30 lecturers), Semester I, After successfully completing this course, students will be able to:, CO1: Explain the application of sampling in biological sciences., CO2: Explain standard Probability distributions., CO3: Explain the concept and types of central tendency., CO4: Explain the concept of correlation and regression with their properties., CO5: Classify the given data., CO6: Graphically represent the given data., CO7: Illustrate the measures of dispersion with examples., CO8: Solve statistical problems., , Savitribai Phule Pune University, , 22

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M. Sc. [I], , Sr., , Name of the topic, , No, , Zoology, , Lectures, allotted, , ., 1., , Introduction:, , (02L), , 1.1 Applications and Uses of Statistics, 1.2 Population & sample, Different types of Sample, 1.3 Exercise & Problems., 2., , Data Classification:, , (3L), , 2.1 Some important terms (Class frequency, class- limits, Class-width, class, mark), 2.2Frequency distribution, Cumulative frequency, Graphical representation of, data (Histogram, Pie-Diagram, Ogive-Curve.), 2.3 Exercise & Problems, 3., , Measures of central tendency:, , (3L), , 3.1 Concept of central tendency, Types of central tendency (Arithmetic mean,, Median and mode) combined mean., 3.2 Partition values (Quartiles, Deciles, and Percentiles), Box plot., 3.3 Exercise & Problems., 4., , Measures of dispersion:, , (4L), , 4.1 Concept of dispersion, absolute and relative measure of dispersion., 4.2 Different measures of dispersion (Range, Quartile- Deviation, Variance and, standard-deviation, Coefficient of Variation) combined variance, 4.3 Exercise & Problems., 5., , Correlation and Regression:, , (5L), , 5.1 Bivariate data, concept of correlation, Types of Correlation, Scatter, diagram, Karl Pearson’s coefficient of correlation and its properties., 5.2 Concept of regression, Linear regression, regression Coefficients and its, properties., 5.3 Exercise & Problems., 6., , Probability and probability distribution:, , (5L), , 6.1 Some important terms (types of experiment, sample Space and types of, sample space, events and types of events.), 6.2 Definition of probability (mathematical and classical) Conditional, Savitribai Phule Pune University, , 23

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M. Sc. [I], , Zoology, , probability. Concept of random variable Univariate Probability Distribution and, its mathematical expectation., 6.3 Some standard probability distributions (binomial, Poisson and normal), their probability distribution, mean, variance, and properties of these, distribution., 6.4 Exercise & Problems., 7., , Test of hypothesis:, , (8L), , 7.1 Some important terms (hypothesis, types of hypothesis, Test, Critical, region, acceptance region, type I error, type II error, level of significance, pvalue), 7.2 Test for mean and equality of two population means, Test for proportion, and equality of two population proportions., 7.3 chi-square test for goodness of fit, Unpaired and paired t test. F test for, equality of two population variances., 7.4 Software used for Statistical analysis, 7.5 Exercise & Problems., , Course Code and Course Name:, ZODP 114 Practical Biostatistics., , (2 Credits- 60 Hours), , Semester I, After successfully completing this course, students will be able to:, CO1: Construct frequency distribution chart., CO2: Graphically represent the given data., CO3: Solve the statistical problems based on Central Tendency, Dispersion,, Correlation andregression., CO4: Apply computer software for statistical analysis., CO5: Solve numerical problems on test of hypothesis using biological data., , Savitribai Phule Pune University, , 24

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M. Sc. [I], , Sr., , Zoology, , ZODP 114 Practical Biostatistics, , No. of, , No, , Practicals, , 1., , Construction of frequency distribution and its graphical representation., , (1P), , 2., , Measures of Central Tendency., , (2P), , 3., , Measures of Dispersion, , (2P), , 4., , Correlation and Regression., , (1P), , 5., , Computation and application of binomial & Poisson probabilities., , (1P), , 6., , Computation and application of normal probabilities., , (2P), , 7., , Test for means and proportions, , (1P), , 8., , Chi-square test of goodness of fit., , (1P), , 9., , Paired and unpaired t- test, F-test., , (2P), , 10., , Statistical analysis with Computer software packages, , (2P), , REFERENCE BOOKS:, 1. Principles and Practice of Biostatistics: Dr J.V. Dixit, 2. Statistical Methods: Snedecor G.W. & Cochran W.G., 3. Statistical Methods: Dixon W.S. and Massey, 4. Biostatistics for the Biological and Health Sciences, 2nd Edition, by Marc M. Triola, Mario F. Triola, Jason Roy,Published by Pearson, Copyright © 2018, 5. Biostatistics: Basic Concepts and Methodology for the Health Sciences,, 10ed, ISV by Wayne W. Daniel, Wiley Publication., Course Code and Course Name:, ZODT 114 Freshwater Zoology., , (2 Credits= 30 lecturers), , Semester I, After successfully completing this course, students will be able to:, CO1: Enlist the diagnostic features of shrimps., CO2: Explain the types of aquatic habitats., CO3: Discuss the aquatic adaptations of common freshwater forms., CO4: Explain the adaptations in freshwater Turtles and Crocodiles., Savitribai Phule Pune University, , 25

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M. Sc. [I], , Zoology, , CO5: Illustrate the physicochemical properties of water., CO6: Demonstrate the effect of pollutants on freshwater bodies, CO7: Justify the presence of zooplanktons and aquatics forms in freshwater bodies., Sr., , Name of the topic, , No., 1., , Lectures, allotted, , Introduction to freshwater habitats., , (02L), , Lotic habitat: Major rivers in India, Lentic habitat: Lakes, ponds, wetlands and ephemeral water bodies. Thermal, stratification in lakes., 2., , Physico-chemical conditions of freshwater and its implications on freshwater, , (7L), , biota., Physical conditions: Water current, water colour, turbidity and temperature., Chemical conditions : pH, carbon dioxide, dissolved oxygen, hardness, acidity, and alkalinity, nitrate, phosphate and sulphates., 3., , General characters of plankton with special emphasis on the characters used in (4L), taxonomy and reproduction:, Rotifera, Copepoda, Cladocera and Ostracoda., , 4., , Introduction to minor phyla:, , (2L), , Gastrotricha, Bryozoa and Tardigrada (water bears)., 5., , Diversity and economic importance of freshwater Crustacea and Mollusca., , (2L), , 6., , Locomotory and respiratory adaptation in freshwater insect orders:, , (2L), , Ephemeroptera,Odonata, Plecoptera, Coleoptera, Diptera and Hemiptera. ., 7., , Diversity and distribution of vertebrates in the freshwater bodies of India. (2L), (Excluding fishes), , 8., , Adaptive physiology of freshwater fauna:, , (6L), , Osmotic and ionic, thermal, respiratory, reproductive and life cycle,, mechanical, locomotory and sensory adaptations., 9., , Anthropogenic impact on freshwater:, , (3L), , Waste and pollution, sewage and silage, mining waste, agricultural chemicals,, industrial outflows and invasions., Eutrophication : Causes and problems., , Savitribai Phule Pune University, , 26

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M. Sc. [I], , Zoology, , Course Code and Course Name:, ZODP 114 Practical Freshwater Zoology., , (2 Credits = 60 lectures.), , Semester I, After successfully completing this course, students will be able to:, CO1: Identify commercially important freshwater fish., CO2: Identify the aquatic adaptations in common freshwater forms., CO3: Prepare the culture of Paramecium and Daphnia., CO4: Estimate the hardness and chloride content in water samples., CO5: Analyze the Zooplanktons from local freshwater bodies., CO6: Evaluate the bio-indicators of pollution in freshwater., , Sr., , ZODP 114 Practical Freshwater Zoology, , No., , No. of, Practicals, , 1., , Estimation of total carbon dioxide and chloride form given water sample., , (1P), , 2., , Estimation of phosphates forms given water sample., , (1P), , 3., , Estimation of total nitrate from given water sample., , (1P), , 4., , Estimation of calcium and total hardness of given water sample., , (1P), , 5., , Estimation of total alkalinity of given water sample., , (1P), , 6., , Collection, preservation and identification of zooplankton from freshwater (2P), habitat. (Prepare permanent slides and Identify up to genus level using, taxonomic key)., , 7., , A qualitative and quantitative analysis of zooplankton from a given freshwater, , (1P), , sample using Lackey’s drop count method/ Sedgwick rafter counting cell., 8., , Identification of economically important freshwater crustaceans and fishes., , (1P), , 9., , Study of locomotory and respiratory adaptations in aquatic insects and larvae, , (1P), , (Ranatra, Notonecta, Gerris, Bellostoma and Dytiscus)., 10., , Study of aquatic and semiaquatic adaptations in amphibians and reptiles., , (1P), , 11., , Estimation of primary productivity with dark and light bottle method, , (1P), , 12., , To prepare and maintain a culture of paramecium, Daphnia and Hydra, , (2P), , 13., , A Compulsory visit to local freshwater body and preparation of report on, , (1P), , physicochemical conditions and faunal organisms., REFERENCE BOOKS:, 1. Limnology: lake and river ecosystem, Robert G. Wetzel. Academic Press,, Savitribai Phule Pune University, , 27

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M. Sc. [I], , Zoology, , 3rd edition., 2. Treatise of Limnology. Hutchinson G. E. John Willy Publication, New, York (3 volumes)., 3. Field Guide to freshwater invertebrates of North America. Thorp and, Rogers. Academic press., 4. Environmental Physiology of Animals. Pat Wilmer, Graham Stone and Ian, Johnston. Wiley-Blackwell; 2nd edition., 5. Current status of freshwater resources of India. Kailash Chandra, Gopi, K.C., Rao D.V., Valarmathi K. and Alfred J.R.B. Zoological survey of, India, 2017., 6. Freshwater Ecology: Concepts and Environmental Applications of, Limnology. Academic press, 2nd edition., 7. Freshwater invertebrates of the United States. Robert Pennak. A WileyInterscience Publication., 8. Freshwater Biology. Whipple and Ward. John Wiley & Sons Inc; 2nd, edition (December 1959)., 9. Freshwater Invertebrates: Ecology and General Biology. Thorp and, Covich. Academic Press, 4th edition., 10. Limnological Methods. Paul and Welch. Mcgraw –Hill publication., 11. Limnological analysis. Wetzel Robert G., Springer Publication., , Savitribai Phule Pune University, , 28

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M. Sc. [I], , Zoology, , Course Code and Course Name:, ZOUP 115 Basic Zoology Lab-1., , (4 Credits- 120 hours), , Semester I, Note: A total of 30 practicals are to be conducted. 10 practicals from each module are to be, conducted. 1 practical is of 4 clock hour duration., After successfully completing this course, students will be able to:, CO1: Identify the developmental stages of chick embryo, cell structures and phases of, cell division, CO2: Identify the grammatical mistakes from the given paragraph and common errors in, written and spoken presentations., CO3:Write a scientific project and research article along with its proof reading., CO4: Demonstrate the working of different microscopes, colorimetric and, spectrophotometric methods,cell fractionation and ligature in Drosophila, larvae,, CO5:Determine the gene distance and order, genotype and phenotype ratios and allelic, frequencies from the given data., CO6: Estimate sugar and protein by suitable biochemical method, and isolate protein, from biological source., CO7: Prepare acid and base solutions of desired strength, buffers, bacterial Culture, chick, embryo culture and Drosophila culture., CO8: Prepare temporary slide of various cells to demonstrate the cell morphology and, cell division, giant chromosome and pedigree analysis chart., CO9: Calculate % retention and % elution of amino acids on given ion exchanger., Sr., , Name of the Practical, , No., , No. of, Practicals, , Module-I Biochemistry and Biochemical Techniques, 1., , Preparation of Acid & Alkali solutions and acid-base titration (Compulsory), , 1P, , 2., , Principle and working of Colorimetry and spectrophotometry, , 1P, , 3., , To prepare Buffers of known pH and molarity and measurement of pH of, , 1P, , various samples, Buffering capacity, 4., , Units and specific activity of enzymes., , 1P, , 5., , Effect of substrate concentration on enzyme activity (Compulsory), , 1P, , Savitribai Phule Pune University, , 29

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M. Sc. [I], , 6, , Zoology, , Determine the concentration of Vitamin C by titration method from various, , 1P, , sources., 7., , Estimation of Sugar (Glucose) by Folin Wu method. (Compulsory), , 1P, , 8., , Isolate proteins by salting out / by adjusting isoelectric point. ( Compulsory), , 1P, , 9., , Estimation of protein by Biuret method method.( Compulsory), , 1P, , 10, , Principle and uses of different microscopes, , 1P, , 11, , Principles of electrophoresis, separation of proteins using Gel, , 1P, , electrophoresis.(Compulsory), 12., , To find out the capacity and nature of a given ion-exchanger. Investigate the 1P, % retention and %elution of amino acids on a given ion exchanger, , 13, , To study the effect of different solvents for a given dye using thin layer, , 1P, , chromatography (Compulsory), 14., , Enzyme isolation and purification by fractionation methods., , 2P, , Module-II Cell Biology and Developmental Biology, 1, , Study of ultrastructure of cell organelles. (Compulsory), , 1P, , 2, , Study of different types of cells. (Compulsory), , 1P, , 3, , Temporary preparation of human cheek epithelial cells. (Compulsory), , 1P, , 4, , Study of different stages of mitosis in suitable material. (Compulsory), , 1P, , 5, , Study of meiosis in Grasshopper testes / Onion flower buds / Aloe vera with, , 1P, , emphasis on all stages of prophase. (Compulsory), 6, , Limits of cleanliness (To check for microbial flora), , 2P, , 7, , Cell fractionation – Nuclei, mitochondria observation, nuclear count., , 2P, , 8, , Mounting of chick embryos and preparation of permanent mounts, , 1P, , (Compulsory), 9, , Filter paper ring method for in vitro culturing of chick Embryo &, , 1P, , observations., 10, , Gross anatomy and histology of chick embryo upto 72 hrs. Brain, heart, lens,, , 1P, , ear development.(Compulsory), 11, , Study of embryonic and post-embryonic development using frog egg as a, , 1P, , model system., 12, , Study of life cycle of Drosophila melanogaster.(Compulsory), , 1P, , 13, , Study of effect of ligature in Drosophila / House fly larva, , 1P, , 14, , Study of regeneration in Hydra/ Planaria., , 1P, , Savitribai Phule Pune University, , 30

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M. Sc. [I], , Zoology, , Study the early developmental stages in any freshwater snail., , 1P, , Module-III Genetics and English in Scientific communication, , 1P, , 1, , Study of sex linked inheritance in Drosophila sp., , 1P, , 2, , Study of monohybrid and Dihybrid ratio in Drosophila sp.(Compulsory), , 1P, , 3, , Determination of gene distances and gene order for a given three-point test, , 1P, , 15, , cross.(Compulsory), 4, , Polytene chromosomes of Drosophila or Chironomous-examination of puff, , 1P, , and bands.(Compulsory), 5, , Estimation of allelic frequencies, heterozygote frequencies in human, , 1P, , populations.(Compulsory), 6, , Pedigree Analysis: Sex-Linked, Autosomal dominant and recessive., , 1P, , 7, , Culturing E.Coli on solid and liquid media, , 1P, , 8, , English vocabulary, word formation, basic grammar-verb, adverb, adjective,, , 1P, , noun, pronoun (Compulsory), 9, , Syntax, paraphrasing and précis writing, synonyms, antonyms, abbreviations., , 1P, , 10, , Spoken English: pronunciation, diphthong, accent, clarity, speed,, , 1P, , punctuation, simplicity and syntax, 11, , Common errors in written and spoken presentation- Tautology, double, , 1P, , negatives and double positives, sequence and tenses, ambiguity, spellings,, jargons., 12, , Outline of a scientific paper; preparation of a research project., , 1P, , 13, , Writing abstracts, conclusion/ summary and acknowledgements, key words, , 1P, , and suggest a suitable title to the given abstract/paper(Compulsory), 14, , Assigning legends to given graphs, figures and captions to given tables,, , 1P, , Deciphering the given pictorals., 15, , Study of proof correction symbols; proof- reading the given text & correcting, , 1P, , the proofs., 16, , How to write materials and methods ,observation section of a research paper., , 1P, , 17, , Write discussion section for the given discussion-less research paperand, , 1P, , writingCitations/ Bibliography (Compulsory), 18, , Oral presentation: Rhythm, style, control, mock presentation of 10 minutes., , Savitribai Phule Pune University, , 1P, , 31

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M. Sc. [I], , Zoology, , M.Sc. Zoology (Semester - 2), Course Code and Course Name:, ZOUT 121: Molecular Biology and Bioinformatics., , (4 Credits = 60 lectures), , Semester II, After successfully completing this course, students will be able to:, CO1: Explain the DNA structure & types, topology, Physical properties; chromatin structure, and organization., CO2: Discuss genome organization, DNA and Protein sequencing with their application in, evolutionary studies., CO3: Explain the mobile DNA elements., CO4: Explain mechanism of DNA damage and repair., CO5: Illustrate the process of DNA replication, transcription, translation and their, regulations., CO6: Illustrate the database tools with their significance., CO7: Schematically represent the processes of central dogma., CO8: Justify the post translational and post transcriptional modifications., , Sr., , Name of the topic, , No., 1., , Lectures, allotted, , DNA structure and topology :-Structure of chromatin, nucleosome, chromatin (5L), organization and remodeling, higher order organization - chromosome,, centromere, telomere, Histones and its effect on structure and function of, chromatin, type of DNA (A,B,Z), , 2., , Physical propertiesof DNA: Tm, hypo and hyper chromicity, solubility,, , (2L), , mutarotation and buoyancy., 3., , Genome organization: C value paradox and genome size, Cot curves, repetitive, , (2L), , and non-repetitive DNA sequence and their importance Cot ½ and, kinetic and, sequence complixicity, satellite DNA., 4., , DNA Replication: DNA replication in E. coli, Origin of replication, , types of (10L), E. coli DNA polymerases, details of replication process, regulation of, replication, connection ofreplication to cell cycle. Different models of, replication for linear and circular DNA replication features of single stranded, , Savitribai Phule Pune University, , 32

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M. Sc. [I], , 3., , Zoology, , Sequence alignment methods, , (4L), , BLAST, FASTA, Significance of sequence alignment, Pairwise sequence alignment (Needleman &Wunsch, Smith & Waterman, methods) Multiple sequence alignment (PRAS, CLUSTALW), 4., , Predictive applications using DNA and protein sequences, , (5L), , Evolutionary studies: Concept of phylogenetic trees, Parsimony and Bayesian, approaches, synonymous and non-synonymous substitutions, convergent and, parallel evolution, Pharmacogenomics: Discovering a drug: Target identification, Protein Chips and Functional Proteomics: Different types of protein chip,, detecting and quantifying; applications of Proteomics, Metabolomics: Concept and applications, REFERENCE BOOKS:, 1. Genes IX, 9th edition (2008), Benjamin Lewin, Publisher - Jones and, BarlettPublishers,Inc., 2. Molecular Biology of the Gene, 5th Edition (2004), James D. Watson,, Tania Baker,, 3. Stephen P. Bell, Alexander Gann, Michael Levine, Richard Lodwick., Publisher -Pearson Education, Inc. and Dorling Kindersley Publishing,, Inc, 4. Bioinformatics - Concepts, Skills, and Applications; S.C. Rastogi&, others; CBS Publishing; 2003., 5. Bioinformatics - A practical guide to analysis of Genes & Proteins;, Andreas D Baxevanis& B F Francis; John Wiley; 2000., 6. Introduction to Bioinformatics; 1st Edition; T K Attwood, D J parrySmith; Pearson Education, 11th Reprint; 2005., 7. Bioinformatics; 1st Edition; C S V Murthy; Himalaya Publishing House;, 2003, 8. Bioinformatics sequence and genome analysis; David W. Mount; Cold, spring harbor laboratory press; 2004, 9. Basic Bioinformatics; S. Ignacimuthu, S.J.; Narosa Publishing House;, 1995, Savitribai Phule Pune University, , 34

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M. Sc. [I], , Zoology, , 10. An Introduction to Bioinformatics Algorithms; Neil C. Jones and Pavel, A. Pevzner; MIT Press, First Indian Reprint; 2005, 11. Bioinformatics - Managing Scientific Data; Zoe Lacroix, Terence, Critchlow; Morgan Kaufmann Publishers (Elsevier Science); 2003 (for, the V unit), 12. Phylogenetics: Theory and Practice of Phylogenetic Systematics; Second, edition; Bruce S. Lieberman; Wiley-Blackwell; 2011, 13. Molecular Evolution: A Phylogenetic Approach; Roderick D.M. Page,, Dr Edward C. Holmes; Well Publishing; 1998, 14. Essential Bioinformatics; JinXiong; Cambridge University Press; 2006, 15. Proteomics - From Protein Sequence to Function; 12 S. R. Pennington,, M. J. Dunn; First edition; Springer publications; 2001, 16. Proteomics; Timothy Palzkill; Springer; 2002, 17. Metabolomics - A Powerful Tool in Systems Biology; Jens Hřiriis, Nielsen, Michael C. Jewett; Springer; 2007, 18. Systems Metabolic Engineering; Dr. Christoph Wittmann, Sang Yup., Lee; Springer; 2012, 19. Bioinformatics (Bios Instant Notes); Second Edition (Special Indian, Edition); T. Charlie Hodgman, Andrew French and David R. Westhead;, Garland Science (Taylor and Francis Group); 2010, 20. Understanding Bioinformatics; Marketa Zvelebil and Jeremy O. Baum;, Garland Science (Taylor and Francis Group); 2008, 21. Bioinformatics Computing – The complete practical guide to, bioinformatics for life scientists; Bryan Bergeron; Eastern Economy, Edition; Prentice-Hall of India Pvt. Ltd., New Delhi; 2003, 22. Bioinformatics; Prakash S. Lohar; MJP Publishers, Chennai; 2009, 23. A text book of Molecular Biology- J.Pal and S. Ghaskadabi, Oxford, Publication- India., 24. Freifelders Essentials of Molecular Biology, Malacinski G.M (2006), (Fourth Edition). Narosa Publishing House, New Delhi., 25. Karp's Cell and Molecular Biology: Concepts and Experiments, 8th, Edition by Gerald Karp, Janet Iwasa, et al. 2016, Wiley Publication, Savitribai Phule Pune University, , 35

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M. Sc. [I], , Zoology, , Course Code and Course Name:, ZOUT 122 Endocrinology and Parasitology., , (4 Credits= 60 lecturers), , Semester II, After successfully completing this course, students will be able to:, Endocrinology:, CO1: Discuss the roles of Pituitary gland and pineal body., CO2: Explain hormonal regulation of biomolecules and mineral metabolism., CO3: Describe the role of osmoregulatory and gastrointestinal hormones., CO4: Explain the role of hormones in moulting, change in body colour of crustaceans; yolk, synthesis in amphibians; insect development., CO5: Explain the hormonal regulation of metabolism., CO6: Illustrate the mechanism of hormone action and role of hormone receptors., CO7: Justify hormones as coordination molecules., CO8: Justify the significance of biological clocks and rhythms., , Parasitology:, CO1: Define the terminologies of parasitology., CO2: Explain the concepts of animal association with examples., CO3: Describe the role of parasites in public health and hygiene., CO4: Explain the morphology and life cycle of common parasites., CO5: Explain the pathogenicity and control measures of common parasites., CO6: Illustrate the process of parasitic infections to human., CO7: Justify the importance of control strategies against parasitic infections., CO8: Justify the significance of vectors and disease transmission., Sr., , Name of the topic, , No., , Lectures, allotted, , Endocrinology:, 1., , Hormones as chemical messenger, types of hormones-protein and non-protein, , (2L), , 2., , Hormone receptors; on the plasma membrane, cytoplasm & nucleus, , (2L), , 3., , Mechanism of hormone action- signal transduction cascade., , (2L), , 4., , Hypothalamic hypophysiotropins, , (2L), , Savitribai Phule Pune University, , 36

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M. Sc. [I], , Zoology, , trends, Melhorn h.,Eds., SprigerVerlag, Beriin., 11. Medical Parasitology, Piakarsky G. L., Springer Verlag, Berlin., 12. Modern Parasitology, Cellular immunological & immunological aspects,, Wyler D. J., Eds.,, 13. Molecular Parasitology: Protozoan parasites and their, MoleculesWalochnik, J, Duchene M, 2016, , Course Code and Course Name:, ZOUT 123 Comparative Animal Physiology & Environmental Biology., Semester II, , (4 Credits= 60 lecturers), , After successfully completing this course, students will be able to:, Comparative Animal Physiology:, CO1: Explain the physiology of processes like digestion, respiration, muscle contraction and, excretion., CO2: Describe the mechanism of thermoregulation in both poikilotherms and homeotherms., CO3: Explain the mechanism of chemical communication in vertebrates., CO4: Comment on the structure and functions of various sense organs., CO5: Illustrate the concept of osmotic regulation in various animals with suitable examples., CO6: Compare the physiology of regulatory mechanisms in various groups of animals., CO7: Justify the survival strategies of organism in varied climatic conditions., CO8: Justify the evolution of various life processes in living forms., Environmental Biology:, CO1:List the endangered, endemic and extinct animal species of India., CO2:Identify various types of natural resources, human impact on these resources, and, common resourcemanagement practices., CO3:Explain the structure and impact of biogeochemical cycles, ecosystems and energy, transformation across trophic levels., CO4: Describe concepts in population ecology and their significance., CO5: Discuss environmental hazards and risks and the socio-economic implications., CO6: Illustrate the impact of climate and anthropogenic factors on biodiversity with, reference, to India., CO7: Illustrate the wildlife management practices and their significance., Savitribai Phule Pune University, , 39

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M. Sc. [I], , Zoology, , CO8: Analyze the impact of lifestyle on the environment and animal life., , Topic, , Name of the topic, , Lecturesallotted, , 1., , Digestion: Physiology of digestion., , (03L), , 2., , Respiration: Respiratory Surfaces: comparison of ventilation associated, , (04L), , No., , with gills and pulmonary respiration. Blood pigment, role in Oxygen, transport.O2 dissociation curves physiological and ecological, significance, CO2, 3., , Muscle contraction: Structure (light & electron microscopic) of the, , (04L), , skeletal muscle, proteins of the myofilaments, nature of actin-myosin, interaction, sarcoplasmic recticulum and role of Ca++ in contraction, 4., , Osmotic regulation: Concepts of osmole, osmolarity and tonicity, ionic, , (04L), , regulation, Hyper-and hypo-osmotic regulators, ureosmotic animals., 5., , Excretion: Basic processes in urine formation, renal function in animals, , (04L), , specially the mammalian kidney, Renal pressure system, Comparative, biochemistry of nitrogen excretion., 6., , Thermoregulation:Biokinetic Zones, tolerance and resistance., , (04L), , Thermobiological terminologies. Compensatory patterns in, piokilotherms. Critical temp, and zone of thermal neutrality. Mechanism, of thermoregulation in homeotherms., 7., , Chemical Communication: Nuerosecretion, neuroheamal& endocrine, , (03L), , organs., 8., , Sense organ: classification & functions (details of photoreception as a, , (04L), , model).Reflexes, Principles of neural integration., , Environmental Biology, 1., , Introduction: Fundamentals of Ecology, Ecosystems: Definition and, (5L), concept of ecosystems, energy flow in ecosystems, Nutritional Flux., Development and evolution of the ecosystems. Biogeochemical cycles,, Food-chains, ecotone, edge effects, ecological niche, and ecosystem, stability., , 2., , Environmental Microbiology: Microbes - classification and their (2L), , Savitribai Phule Pune University, , 40

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M. Sc. [I], , Zoology, , applications in the environmental sciences. Cultivation and growth of, microorganisms. Microorganisms and their association with man,, animals and plants. Microbes as anti-microbial agents., , 3., , Biomes and Habitat Diversity: Classification of biomes, major biotic, , (2L), , elements of each biome and their characteristics. Human impact on the, natural environment., 4., , Biological diversity of India: Definition and nature, India's, , (4L), , biogeographically history, physiography, climate and its impact on, biodiversity. Indian forest and vegetation types and diversity of flora and, fauna., 5., , Population and Community Ecology, , 6., , Wetlands Forests and Semi-Arid Habitats of India: Definition and types (3L), , (2L), , of wetlands, important wetlands and their conservation issues. Forests, and semi-arid habitats and their distribution in India, ecological status of, forests and arid lands, and their conservation., 7., , Endangered, Endemic and Extinct Species of India: Threatened species, , (4L), , categories of IUCN, threatened species of animals and the reasons, Red, data book, Biodiversity hotspots of India., 8., , Wildlife management and conservation. Protected Areas Network in, , (6L), , India: Goals of management, Strategies for planning. Factors influencing, wildlife management such as habitats, population, behaviour, foodhabits, health, etc., tools for data collection and analysis. Human land-use, and wildlife management units, important projects for the conservation, of wildlife in India, Role of local communities in wildlife management., , 9., , Introduction to human animal conflict., , (2L), , REFERENCE BOOKS:, 1. Comparative animal physiology, Clifford Ladd Prosser, John, Wiley & Sons, 2. Animal physiology, Richard W. Hill, Gordon A. Wyse. Harper, and Row, Savitribai Phule Pune University, , 41

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M. Sc. [I], , Zoology, , 3. Comparative animal physiology, Philip Carew Withers, Saunders, College Pub., 1992, 4. Fundamentals of Ecology: E. P. Odum, 5. Modern concepts in Ecology: H: D. Kumar, 6. Microbes, Man and Animals: The Natural History of Microbial, Interactions: Linton, A. H. andBurns, R.G. John Wiley and Sons., 7. Elements of Microbiology: Pelczar, M.J. and Chan ECS,, McGraw Hill., 8. General Microbiology: Stainer, R.Y, Adelberg, EA and, Ingraham, J.L. . Macmillan Press., 9. Microbial Methods for Environmental Biotechnology: Grainer,, J.M. and Lynch, J.M. . Academic Press., 10. Microbiological Methods for Environmental Scientists and, Enginners, 11. Gaudy, A.F. and Guady, E.T. McGraw Hill., Course Code and Course Name:, ZODT 124: Metabolic Pathways.(2 Credits= 30 lecturers), Semester II, CO1: Define basic terminologies of metabolic pathways., CO2: Explain the laws of thermodynamics, concept of free energy and ATP as currency, molecule., CO3: Describe the Concepts and regulation of metabolism., CO4: Discuss the oxidation of fatty acids and its significance., CO5: Illustrate the electron transport chain and oxidative phosphorylation., CO6: Illustrate the reactions, energetics and regulation of glycolysis, glycogen biosynthesis,, TCA cycle, Purine and Pyrimidine metabolism, CO7: Write the general reactions of various metabolic pathways., CO8: Justify the role of enzymes in metabolism, , Savitribai Phule Pune University, , 42

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M. Sc. [I], , Sr., , Zoology, , Name of the topic, , Lectures, , No., 1., , allotted, Basic law of thermodynamics, internal energy, enthalpy, entropy, concept of free (4L), energy, redox potentials, high energy compounds, structure and function of ATP., , 2., , Concepts of metabolism, Metabolic pathways-Catabolic and anabolic, regulation of, , (2L), , metabolic pathways, 3., , Carbohydrate Metabolism, , (7L), , 1. Steps of Glycolysis (EMP Pathway)., 2. PFK, 3. Regulation of Glycolysis., 4. Glycogenesis, Glycogenolysis and Glyconeogenesis, 5. Inborn errors of metabolism- Von- Gerkes disease, Tarui’sdisease,, Galactosemia, Maple syrup urine, 4., , Electron transport chain and oxidative phosphorylation., , 5., , Citric acid cycle: Detailed study, energetics, regulation and significance, Role of (4L), , (3L), , PDH., 6., , Oxidative degradation of amino acids: transamination, oxidative deamination., , (2L), , 7., , Biosynthesis of purine and pyrimidine nucleotides, , (3L), , 8., , A Lipid metabolism: Introduction, oxidation of even chain saturated fatty acids, (5L), oxidation of unsaturated fatty acids, oxidation of odd chain fatty acids, omega (ɷ)oxidation of fatty acids, Ketogenesis. Transport of Fatty Acids., , Course Code and Course Name:, ZODP 124: Practical in Metabolic Pathways., , (2 Credits- 60 Hours), , Semester II, After successfully completing this course, students will be able to:, CO1: Identify the common diseases/conditions caused due to errors in metabolism., CO2: Explain the principle of Colorimetry and Spectrophotometry., CO3: Use the basic equipment in biochemistry lab., CO4: Illustrate the enzyme activity from suitable material., CO5: Demonstrate the effect of various physical and chemical factors on enzyme, activity., CO6: Demonstrate the absorption studies of biomolecules., Savitribai Phule Pune University, , 43

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M. Sc. [I], , Zoology, , CO7: Estimate the concentration of cholesterol, uric acid, amino acids and starch., CO8: Separate biomolecules by chromatographic methods., Sr., , ZODP 124: Practical in Metabolic Pathways., , No., , No. of, Practicals, , 1., , Estimation of Starch.(Compulsory), , (1P), , 2., , Separation amino acids by paper chromatography, , (2P), , 3., , Estimation of uric acid in wall Lizard excreta/ Human blood any other suitable, , (1P), , material.(Compulsory), 4., , To find absorption spectrum of haemoglobin, BSA, Tyrosine., , (1P), , 5., , Estimation of Protein by Lowry et al Method.(Compulsory), , (1P), , 6., , Estimation of Sugar by DNSA method., , (1P), , 7., , Separation of amino acids by TLC (Compulsory), , (1P), , 8., , Estimation of free amino acids by Ninhydrin method.(Compulsory), , (1P), , 9., , Estimation of cholesterol., , (1P), , 10., , Study α-Amylase Activity in Germinating Seeds., , (2P), , 11., , Determination of saponification value of a fat., , (1P), , 12., , Study the effect of pH and temperature on enzyme activity., , (1P), , 13., , Study of human diseases caused due to errors in metabolic pathways with the help, , (1P), , of pictures/charts/models/laboratory reports., REFERENCE BOOKS:, 1. Biochemistry, 3rd Ed. (2005), Voet Donald and Voet Judith G. John,, Publisher: Wiley & sons, New York., 2. Biochemistry 6th Ed, (2007) Berg Jeremy, Tymoczko John, StryerLubert,, Publisher: W. H. Freeman, New York., 3. Lehninger’s Principles of Biochemistry, 4th edition, (2005) Nelson D. L. and, Cox M. M. W. H. Freeman & Co. NY., 4. Biochemical Calculations, 2nd Ed., (2010) Segel Irvin H., Publisher: John, Wiley and Sons, New York., 5. Enzymes: Biochemistry, Biotechnology & Clinical chemistry, (2001) Palmer, Trevor, Publisher: Horwood Pub. Co., England., 6. Biochemistry, Geoffrey Zubay, William C Brown Pub; 4th edition (June, 1999), 7. Principles and Techniques of Biochemistry and Molecular Biology, 6th, Savitribai Phule Pune University, , 44

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M. Sc. [I], , Zoology, , edition (2008), Keith Wilson and John Walker, Publisher–Cambridge, University Press., 8. Biochemical Methods, 2018, S.Sadashivam, New Age International Pvt Ltd, Publishers; Third edition., 9. An Introduction to Practical Biochemistry,2017, David Plummer, McGraw, Hill Education; 3th edition., 10. Medical Biochemistry,2018- John W Baynes and Marek H. Dominiczak,, Publisher- Elsevier; 5thedition., 11. Harper's Illustrated Biochemistry, 2018, Victor W.Rodwell, David A.,, Bender, Kathleen M.,Botham, Peter J.,Kennelly, P. AnthonyWeil, McGrawHill; Illustrated edition,, 12. Principles of Medical Biochemistry-2016, Gerhard Meisenberg, William H., Simmons,Elsevier; 4th edition., Course Code and Course Name:, ZODT 124: Ichthyology(2 Credits= 30 lecturers), Semester II, After successfully completing this course, students will be able to:, CO1: Identify the common fishes in India., CO2: Explain the general characters and evolution of fishes., CO3: Explain the fish morphology and anatomical modifications., CO4: Illustrate the physiology of reproductive and endocrine organs in fish., CO5: Discuss the signs, symptoms and control measures of common diseases in fish., CO6: Justify the role of respiratory and excretory organs in survival of fishes., CO7: Classify fishes upto order level., CO8: Setup aquarium and manage it., , Savitribai Phule Pune University, , 45

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M. Sc. [I], , Topic, , Name of the topic, , No., 1., , Zoology, , Lecture, allotted, , Introduction, general characteristics, evolutionary succession and fossil history (3L), of fishes. The early evolution of fishes: Chondrichthian fishes - Sharks, Skates, and Rays., , 2., , Eschmeyer’s classification of fishes and diagnostic characters up to orders, , (4L), , (one major order from each class)., 3., , External morphology, body form, appendages, pigmentation, skin and scales., , (1L), , 4., , Food and feeding habits, Digestive system and its anatomical modifications., , (2L), , 5., , Respiratory mechanism: Respiratory gills and lungs., , (2L), , Accessory respiratory organs: Origin of air breathing organs; skin,, buccopharynxopercular cavity and air bladder., 6., , Circulatory system: Heart and accessory pumps., , 7., , Excretion and Osmoregulation: Glomerular and aglomerular kidneys; (3L), , (2L), , Nitrogen (ammonia, urea, TMAO) excretions. Water and salt balance in stenoand euryhayline fishes. Role of skin and gills., 8., , Reproduction: Structure of gonads, gametogenic cycles; spawning., , 9, , Nervous system and Sense organs: Organization of the central and peripheral (3L), , (2L), , nervous systems. Olfactory, taste buds, touch receptors, photoreceptors, lateral, line and internal ear, 10, , Endocrine system: Pituitary gland, urohypophysis, adrenal gland, gonads, and (2L), thyroid gland., , 11, , Fish pathology:, , (2L), , Signs of sickness and effects on fish; Pathological procedure for diagnosis of, fish diseases,Symptoms and control measures of viral, bacterial, fungal,, protozoan, worm and crustacean diseases., 12, , Ornamental Fish production and management:, , (4L), , World trade of ornamental fishes. Different varieties of exotic and indigenous, ornamental fishes. Principles of a balanced aquarium. Fabrication, setting up, and maintenance of freshwater aquarium. Water quality management. Water, filtration system – biological, mechanical and chemical. Types of filters., Aquarium plants and their propagation methods. Lighting and aeration., Aquarium accessories and decoratives. Aquarium fish feeds. Dry, wet and live, Savitribai Phule Pune University, , 46

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M. Sc. [I], , Zoology, , feeds. Breeding and rearing of ornamental fishes. Common diseases and their, control. Conditioning, packing, transport and quarantine methods. Trade, regulations and wild life act in relation to ornamental fishes., , Course Code and Course Name:, ZODP 124: Practical in Ichthyology (2 Credits – 60 hours), Semester II, Sr. No., , Name of the Practical, , No.ofPracticals., , 1., , Study of fish evolution with the help of charts/models/Pictures., , (1P), , 2., , General external characters, fins and scales (permanent slides & temporary (1P), preparations and submission during examination)., , 3., , Classification of fishes (one example from each order); use of diagnostic keys., , (2P), , 4., , Length-weight relationship, condition factor, gonado-somatic index of any one, , (1P), , fish species., Adaptations of fishes (adhesive organs, accessory respiratory organs,, , 5., , (1P), , stomachless fishes, spiral valve, electric organs and sense organs.), 6., , Study of Weberian ossicles in Heteropneustes/ Labeo., , 7., , Anatomical observations, demonstration and detailed explanation of fish in (1P), , (1P), , general, Digestive, urino-genital system, Endocrine glands of carp/ catfish/, Tilapia., Identification of fish developmental stages: egg, spawn, fry fingerling and, , 8., , (1P), , adult., Cranial nerves (V, VII, IX & X) and eye ball musculature and innervations in, , 9., , (1P), , Scoliodon/ carp/ catfish., 10., , Histology of digestive, respiratory, excretory, reproductive and endocrine, , (1P), , organs., 11., , Study of common diseases in fish their diagnosis and control strategies., , (1P), , 12., , Setting up of an aquarium and its management and study of breeding, , (2P), , behaviour of gourami, Siamese fighting and swordtail., 13., , Visit to fish farm/ fish breeding centre/fish market and preparation of detail, , (1P), , visit report., , Savitribai Phule Pune University, , 47

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M. Sc. [I], , Zoology, , REFERENCE BOOKS:, 1. Bal D. V. & K. V. Rao (1984). Marine Fisheries. Tata McGraw-Hill, New, Delhi., 2. Bone Q., N. B. Marshall & J. H. S. Blaxter (1995). Biology of Fishes,, Edn.2, Blackie, Academic % Professional (Chapman & Hall), London., 3. C. Vandujn. Diseases of fishes – Narendra Publishing House, New Delhi., 4. Hoar W. S. & D. J. Randall (1969). Fish Physiology. Vols. I onwards,, Academic Press, New York., 5. Jayaram K. C. (1981). The freshwater fishes of India. Pakistan,, Bangladesh, Burma and Sri Lanka- A Handbook. Zool. Survey of India,, Academic Press, New York., 6. Khanna, S. S. (1984). An Introduction to Fishes. Central Book Depot.,, Allahabad., 7. Lagler K. E., J. E. Bardach, R. R. Miller D.R.M. Passino (1977)., Ichthyology, Edn.2, Wiley, New York., 8. Rahul P. Parihar. Textbook Of Fish Biology & Indian Fisheries, 9. Talwar P.K. & A.G. Jhingran (1991). Fish and Fisheries of India and, Adjacent Countries, Vols. I & II. Oxford & I.B.H., New York., 10. Wake, M.H. (Ed.) (1979). Hyman’s Comparative Vertebrate Anatomy., Edn.3, University of Chicago Press, Chicago, 11. Wedemeye G. A. Environmental stress and fish diseases –Narendra., Publishing House., 12. William N. Eschmeyer (2019). Eschmeyer'sCatalog of Fishes (Vol. 1, 2 &, 3). Published by the California Academy of Sciences, San Francisco ©, 1998., , Savitribai Phule Pune University, , 48

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M. Sc. [I], , Zoology, , Course Code and Course Name:, ZOUP 125 Basic Zoology Lab-2(4 Credits, 120 hours), Semester II., Note: A total of 30 practicals are to be conducted. 10 practicals from each module are to be, conducted. 1 practical is of 4 clock hour duration., After successfully completing this course, students will be able to:, CO1: Identify the various parasites and parasitic stages of common parasites, nitrogenous, wasteproducts of animals, feshwater planktons and slides of endocrine glands., CO2: Explain the principle and significance of gonadectomy, thyrodectomyand, pancreactomy., CO3: Demonstrate the role of eye stalk and insulin in sugar level in crab., CO4: Demonstrate the retro cerebral complex in cockroach., CO5: Demonstrate the RBCs of common vertebrates and effect of various osmolarities., CO6: Demonstrate the effect of body size, oxygen consumption and Insulin on aquatic, animal., CO7: Determine the bleeding and clotting time, heartbeat of crab, species richness in, selected area, physico- chemical properties of soil and water., CO8:Perform Sterilization of lab equipment, prepare microbial culture, Isolate Bacterial,, liver DNA and RNA from given sample, quantify and resolve them using, electrophoretic procedures, analyse protein sample by PAGE and SDS PAGE and, construct phylogenetic tree using tools inbioinformatics., , Sr., , Name of the Practical, , No., , No. of, Practicals, , Module-I Molecular Biology and Bioinformatics, 1., , Lab Safety Techniques and sterilization., , 1P, , 2., , Isolation of bacterial DNA and estimation by UV spectrophotometry, , 2P, , (Compulsory), 3., , Isolation of Liver DNA and quantification, Agarose gel electrophoresis of, , 2P, , isolated DNA.(Compulsory), 4., , Isolation of RNA and agarose gel electrophoresis., , 1P, , 5., , Concept of biological database, gene and protein search by BLASTA and, , 1P, , Savitribai Phule Pune University, , 49

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M. Sc. [I], , Zoology, , Module-III Comparative Animal physiology and Environmental Biology, 1, , Study of nitrogenous waste products of animals from different habitats., , 1P, , (Compulsory), 2, , Study of RBCs in different vertebrates and in different physiological, , 1P, , conditions., 3, , Study of relation of Body size and oxygen consumption in aquatic animals, , 1P, , (crab/fish). (Compulsory), 4, , Estimation of sugar in rat/crab/human blood. (Compulsory), , 1P, , 5, , Determination of bleeding time & clotting time of human blood., , 1P, , (Compulsory), 6, , Determination of the heart beat in the crab-effect of temperature & ions., , 1P, , 7, , Effect of eye stalk ablation on glucose in the haemolymph of the crab., , 1P, , 8, , A visit to aquatic ecosystem and methods for water and plankton collection., , 2P, , (Compulsory), 9, , Plankton identification and quantification from river / lake water samples., , 2P, , (Compulsory), 10, , Vegetation studies by line, quadrates and belt transect methods and their, , 2P, , analysis., 11, , Preparation of media for microbial culture, Isolation and culturing of 2P, microbes from soil/water samples. (Compulsory), , 12, , Water analysis for physico-chemical characteristics.(Compulsory), , 1P, , 13, , Physico-chemical analysis of soil. (Compulsory), , 1P, , Note: Latest edition of the recommended books may also be used, , Savitribai Phule Pune University, , 51