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-32Practical Examination :, Distribution of Marks :, 1. Determination of physical properties of seeds of different crops, 2. Identification of farm machine and their use, 3. Study of operations of seed treatment equipment, 4. Identify and describe equipments A,B,C,D,E,, 5. Submission of field Report, 6. Submission of seed specimen & Viva -voce, 7. Record book, , Marks 50, 10, 10, 05, 10, 05, 05, 05, , Books Recommended :, 1. Hand book of Agriculture, Indian Council of Agricultural Research,Krishi Bhavan, New Delhi, 2. Farm Power and Machinery Management, Vth edition, 10WA State, U.S.A. Hunt, D,1968, 3. Farm Management Decision, Operation Control.John E Kadlec, Prentice Hall, Inc Englwood, Chiffs, New, jersey, U.S.A., 4. Fundamentals of farm Management S.S. Joshi and T.R. Kapur, Kalyani Publishers, India, Ludhiana., 5. Fundamentals of farm Management A.S.Kahlon and Karam Singh, Kalyani Awed Publishers PVT.Ltd. 13/14, Asaf Ali Road New delhi/Madras/Bombay/Calcutta/Bangalore., 6. Economics of farm Production and Management, V.T. Raju and DVS Rao, IBH Publishing Co Pvt.Ltd.New, Delhi., 7. Agricultural Marketing in India, S.S.Achary Oxford and I.B.H., New Delhi., 8. Seed Technology - R.L.Agrawal, B.SC. FINAL, SEMESTER-V, 10 : ZOOLOGY, There shall be the following paper and practical for B.Sc. Part-III Semester V examination. The syllabus is, based on 6 theory periods and six practical periods per week (Total 75-80 theory sessions and 25 practical sessions, during the complete semester). There shall a compulsory theory paper of 3 hours duration, as stated below and a, practical examination extending for five hours. Every examinee shall offer the following paper of 100 marks (80 for, written examination and 20 marks for internal assessment) and a practical examination of 50 marks. Candidates are, required to pass separately in theory and practical examination., Theory -5 S-ZOOLOGY: (ANIMAL PHYSIOLOGY AND ECONOMIC ZOOLOGY), 1) Written examination……….., Internal assessment…..……., 2) Practical:, , Marks Allotted, 80, 20, 50, ---------------------------Total: ……, 150 Marks, , Paper 5 S-ZOOLOGY, (ANIMAL PHYSIOLOGY AND ECONOMIC ZOOLOGY), Max. Marks - 100 Total, , Period - 75, , UNIT I, Respiration:, Structure of respiratory organs: Gills and Lungs, Mechanism of respiration: regulation of ventilation in lungs, exchange of gases at respiratory surface,, Respiratory pigments in animals: Haemoglobin, Haemocyanin, Haemerythrin, chlorocruorin., Transport of gases: O2 and CO2 transport, Neurophysiologic control of respiration,, Circulation:, Blood : Definition and its constituents, functions of blood., Heart: Structure of human heart, pace maker, Cardiac cycle., Blood coagulation factors, blood groups A, B, O system and Rh-factor., UNIT II, Muscle Physiology:, Types of Muscles: striated, non-striated and cardiac muscles, E.M. Structure and Chemical Composition of striated muscle, Neuromuscular junction., Mechanism of muscle contraction by Sliding filament theory, Physical and Chemical changes during muscle contraction: muscle twitch, tetanus, isometric and isotonic, contraction, summation of Stimuli, all or none law, fatigue, rigor mortis., UNIT III, Nerve Physiology:, Neuron: E.M. Structure of nuron andTypes : Myelinated and non-Myelinated nerve fibres., Conduction of Nerve impulse, Resting potential, initiation and propagation of action potential, Saltatory, transmission, Neurotransmitters (Acetylcholine, dopamine, GABA, Serotonin, Epinephrine, NorEpinephrine), Synapse and synaptic transmission
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-33Chemical co-ordination:, Endocrine system: Hormones and their physiological roles ofPituitary, Thyroid, Parathyroid, Adrenal, Islets of Langerhan’s,, Hormonal disorders: Dwarfism, Gigantism, Acromegaly , Goiter, Myxoedema, Cretinism, Osteoporosis ,, UNIT IV, Reproductive Physiology:, Estrous and menstrual cycle, hormonal control of reproduction in males and female,, Structure and physiology of mammalian Placenta., Homeostasis and conservative regulation:, Osmoregulation and ionic regulation in aquatic animals. Osmoregulation in terrestrial animals, Ammonotelism, ureotelism and uricotelism., Thermoregulation in Poikilotherms and Homeotherms., UNIT –V, Agricultural Zoology: Economic importance of Insects, Beneficial insects – Spider, Mantis, Ladybugs, Damsel bug, Mealybug destroyer, Soldier beetle,, Green lacewing, Syrphid fly, Tachinid fly, Ichneumon wasp and Trichogramma wasp., Harmful Insects –Stired food grain pests, their injuries and control, Pests of,– Cotton, Sugarcane and Jowar. Damage and Control, Economic importance of Rodents, Snakes, Owls and Bats., Apiculture - Sericulture Unit –VI., Aquaculture, Aquaculture–: definition, scope, importance and present status in India., Fresh water fish culture: types of fish ponds:Nursary, rearing and stocking, design and construction of fish, pond, fertilizers used for fish development., Hatching Happas, Chinease Circular Hatchery, CIFE, Mumbai, hatching model, Induced breeding and, hypophysation, Modern drugs used in fish breeding., Freshwater system: monoculture, polyculture, integrated aquaculture, cage culture, pen culture . Fish, products and byproducts: Fish liver Oil, Fish body oil, Fish manure, Fish leather, Practicals:, 1. Detection of blood groups in human being., 2. Differential counts of blood., 3. Estimation of hemoglobin percentage with the help of haemometer., 4. R.B.C. count., 5. W.B.C. count., 6. Preparation of haemin crystals, 7. Measurement of blood pressure., 8. Action of salivary amylase on starch., 9. Qualitative detection of digestive enzymes in cockroach and crabs., 10. Qualitative detection of nitrogenous waste products in fish (Ammonia, urea, uric acid) in fish tank water, frog, tank water, bird excreta and urine of mammal., 10. Use of, kymograph unit, Respirometer., 11. Collection and identification of Insect Pests of local crops, and predator insects., 12. Life Cycles of Honey bee, Lac insect, Silk Moth., 14. Histological Slides of major organs of Respiratory systems, circulatory system, Nervous system, Different, types of Muscles, Endocrine glands, testis, ovary, placenta., 15. Study of locally available fishes, Indian major carps, Exotic carps, Common carp., 16. Dissection of Digestive system, Urinogenital system, Pituitary Gland of locally available fishes, Distribution of marks for practical examination :, Time: 5 Hrs., Marks, 01. Physiological Expt., a) Major………………………………….. ., 10, b) Minor ………………………………….. ., 05, 02. Economic Zoology, a) Spotting (A-E) Based On Unit V and VI, 10, b) Description and Comments on Topic from Unit V and VI / Dissection, 10, 04. Class record duly signed by teacher in charge and certified by H.O.D., 05, 05. Study tour diary and collection of animals., 05, 06. Viva - voce, 05, ------------------------------------------------------------------------------------------------------------------Total Marks, 50, -------------------------------------------------------------------------------------------------------------------
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-34REFERENCES, 1. Prosser and Brown : Comparative Animal Physiology, 2. Hisotlogical Slides of Respirator systems, circulatory system, Muscles, Nervous system Endocrine glands,, Gonads, placentae, 3. Guyton : Physiology, 4. Best and Taylor : Physiological basis of Medical practice, 5. C Hoar, W.S.. General and comparative Physiology. Prentice Hall of India., 6. Lehninger. L.. Biochemistry. W.H. Freeman & co., 7. Nagabushnam, R.. Animal physiology. S.Chand & co., 8. Martin, D.W. P.A. Mayes and W.W. Rodwell,.Harper’s Review of Biochemistry lange Medical Publications., 9. Prosser, C.L. and F.A.Brown Comparative Animal physiology. W.B. Suanders., 10. Rama Rao, A.V.S.S.. Biochemistry. UBSPD., 11. Stryer. L. Biochemistry Wiley International, 12. Verma, P.S. and V.K. Agarwal.. Animal physiology. S.Chand & co., 13. Wilson, J.A., Principles of Animal Physiology, Macmillan, 14. Chatterjee, C.J; Human Physiology(Vol-I and II), 15. Economic Zoology, G.S. Shukla, V.B. Upadhyay (2006), 16. Text Book of Applied Zoology, Pradip. V Jabde (2005)., 17. Mac E. Hadley: Endocrinology, Prentice Hall, International Edition, 2000, B.SC. FINAL, SEMESTER-VI, ZOOLOGY, There shall be the following paper and practical for B.Sc. Part-III Semester VI examination. The syllabus is, based on 6 theory periods and six practical periods per week (Total 75-80 theory sessions and 25 practical sessions, during the complete semester). There shall a compulsory theory paper of 3 hours duration, as stated below and a, practical examination extending for five hours. Every examinee shall offer the following paper of 100 marks (80 for, written examination and 20 marks for internal assessment) and a practical examination of 50 marks. Candidates are, required to pass separately in theory and practical examination., Theory -6 S-ZOOLOGY : (MOLECULAR BIOLOGY AND BIOTECHNOLOGY), Marks Allotted, 1), Written examination……….., 80, Internal assessment, ………., 20, 2), Practical:, 50, ----------------------Total: 150 Marks, Paper- 6 S-ZOOLOGY, (MOLECULAR BIOLOGY AND BIOTECHNOLOGY), Max. Marks - 100, Total Period - 75, UNIT – I :, Genetic material-definition, Experiments to prove DNA as genetic material:Griffiths, transformation experiments with bacteriophage infections, Avery and co-workers experiments, and, Hershey and Chase experiment. Chemistry and types DNA(A,B,Z)Mitochondrial DNA;, Chemistry, types and function of RNA: mRNA, tRNA and rRNA and Non Genetic RNA., UNIT - II :, , DNA replication: semi conservative method; experiment by Messelson and Stahl., Concept of genes, one gene one enzyme hypothesis, one gene one Polypeptide theory.; A brief, account of Concept and action of cistron, split genes, overlapping genes, jumping genes, Genetic, diseases: Spinocerebellar ataxia., , UNIT–III :, , Genetic code and its features, Protein synthesis-transcription and processing of mRNA,, translation-different steps, Gene regulation: (promoter and operator), Operon models, and Lacoperon model of E.Coli. Genetic regulation in Eukaryotes-Britten Davidson Model., , UNIT–IV :, , Mutation: Definition-mutation theory of DeVries-different types of mutations, - molecular basis of, mutation: substitution and frameshift mutations, chromosomal aberrations-structural(deletion,, addition, inversion and translocation),numerical (euploidy and aneuploidy). Natural and induced, mutations-significance of mutations., DNA repair process., Polymerase chain reaction (PCR). Southern, Northern and Western blotting techniques, DNA, finger printing., , UNIT – V :, , Biotechnology:. Genetic Engineering: Recombinant DNA technology and gene cloning-enzymes, in recombinant DNA technology, Splicing and cloning of genes, vectors (plasmid and phage, vectors), gene Transfer. Somatic cell hybridization, hybridoma technology, and monoclonal, antibodies. Practical applications and suspected hazards of biotechnology and genetic engineering, in animals., Immunology: Introduction to immune system: Innate and adaptive immunity, Types and, production of immune cells ; Complement system., Humoral Immunity: Antigen and haptens, Antibody: types function, and production., Cell mediated immunity: T-cell receptors, T helper cell and lymphocyte activation, Role of cytotoxic T-cell..ELIZA Technique RIA., , UNIT-VI, , :
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-35Practicals:, 1. Microtechnique scope and importance., 2. Preparation of fixatives - Alcohol, Acetone, Formalin, Bouin’s fluid, Cornoy fluid, Formal sublimate., 3. Collection, of various tissues of available animal for micro-technique,, 4. Preparation of Alcoholic grades, dehydration and clearing of tissues,, 5. Use and care of Oven,, 6. Embedding and block making, trimming of block., 7. Use and Care of different types of Microtome., 8. Honing and stropping Knives, 9. Section cutting and spreading,, 10. Preparation of various stains -Borax carmine Acetocarmin, Aceto-orcein, Haematoxyline, eosin., 11. Staining of the sections, (Double Staining), mounting., 12. Camera Lucida. Use and Drawings, 13. Oculomicrometer scale/ similar micro-measurements use, 14. Models of PCR, Southern blotting., 15. Vital Staining of mitochondria by using Janus, Green B stain., 16. Extraction of DNA/ RNA from liver/ Spleen., 17. Methods for the microbiological examination of water and foods., Distribution of marks for practical examination :, Time: 5 Hrs. Marks, 01 Microtechnique., a) Trimming and Section cutting of the Paraffin blocks, 05, b) Spreading of ribbons., 05, c) Staining of the given slide /, 10, c) Use of camera Lucida/ Ocular micrometer sacales, 05, 02. Biotechnology experiment., 10, 03. Permanent slides submitted by the examinee (5 Slides), 05, 04. Class record duly signed by teacher in charge and certified, 05, By H.O.D., 05. Viva - voce, 05, -----------------------------------------------------------------------------------------------------------Total Marks, 50, -----------------------------------------------------------------------------------------------------------REFERENCES, 1. Friefelder. D. Microbial Genetics; Narosa Publishing, New Delhi., 2. Goodenough, U. Genetics. Saunders Coolege Publishing International, New York., 3. Klug, W.S. and M.R.Cummings. Concepts in Genetics; Charles E.Merrill Publishing Co. London., 4. Kumar, H.D. Molecular biology and biotechnology. Vikas Publishing House, New Delhi., 5. Lewin, B.. Gene VI . Wiley Eastern Ltd., New Delhi., 6. Rothwell, N.V. Human Genetics. Prentice Hall of India, New Delhi., 7. Sinnott, E.W.L.C.Dunn, and L.C.Dobzhansky, T. 1985. Principles of Genetics. Tata McGraw Hill. New Delhi., 8. Stern, C. Principles of Human genetics. S.Chand & Co. New Delhi., 9. Verma, P.S. and V.K. Agarwal.. Genetics. S.Chand & Co. New Delhi., 10. Balasubramania, D., Concepts in Biotechnology. Unversity Press (India) Ltd., Hyderabad., 11. Chopra, V.L and A.Nasim,. Genetic Engineering and Biotechnology. Oxford & I BH, New Delhi., 12. Dharmarajan, M. Genetic Engineering S.viswanathan & Co., 13. Dubey, R.C.1995. Text book of Biotechnology. S.Chand & Co., 14. Glick, B.R. J.J. and Pastermak. 1998. Molecular Biotechnology. SSM Press, Washington., 15. Gupta, P.K. Elements of Biotechnology. Rastogi Publications, Meerut., 16. Jogdand, S.N. Advances in Biotechnology. Himalaya Publishing, New Delhi., 17. Kumar, H.D.. A text book on Biotechnology. East West Affiliated Press Ltd., 18. Proter, D.G. Ethical scores for animal experiments. Nature 356: 101-102., 19. Primrose, S.M. Modern Biotechnology. Blackwell Scientific Publishers, Oxford., 20. Trevan, M.D. Biotechnology: The Biological principles. Tata McGraw Hill Publishing Co., New Delhi., 21. Trehan, K. Biotechnology. Wiley eastern ltd., New Delhi., 22. Vijayaraman, K.S.Chellammal and P.Manikili. 1998. Uyiriyathozhilnutpam. Chimeeraa, Tiruchy., 23. AM. Pearson & TA Gillett (1996) Processed Meats,, 24. W.J. Stadelman, V.M. Olson, GA. Shemwell & S. Pasch S., 25. Egg and poultry meat processing,, 26. Bremner (2002) Fish as Food, Vol 1 & 2, HA, 27. Ivan Roitt: Essential Immunology (6th Ed.) Oxford, Backwill, Science publication London., 28. Elgert: Immunology understanding the immune system, John Willy & Sons, Inc. publication, New York.
