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27, Select the wrong statement,, , 28., , 29,, , 30., , The presence of chromogenic substerte gives: blue, colour colonies, ifthe plasmid in the bacteria does, not have an insert., , Retroviruses inaninutls have the ability to transform,, normal cells into cancerous cells,, te) In vinjection, cells are bombarded with high, velovity microparticles of gold or tungsten coated, with DNA., , Since DNA is a hydrophilic molecule it cannot pass, through cell membranes,, , DNA is a negatively charged molecule., , (Kerala PMT), , jw, , (by, , , , , aw), , «), , ‘The function ofa selectable marker is, , (a) climinating transtormants and permitting nontransformants, , identify ov? site, , elimination of non-transformants and permitting, transformants, , to destroy recognition sites., , (b), {c), , (d), , With respect to DNA fragmentation, Statement A : Gel electrophoresis and elution are (wo, important processes., Statement B : After staining with ethidium bromide it, has to be exposed to U.V. light., {a) Only A is correct., (b) Both A and B are correct statements,, (c) Only B is correct., (d) Only A is correct and B is not correct., (Karnataka CET), , The enzyme used for the purpose of joining a novel gene, with host DNA is, (a) endonuclease, , (c) ligase, , (Karnataka CET), , (b) helicase, (d) polymerase. (UP CPIAT), , 2014, , 3., , 32., , Which vector can clone only a small fragment of, DNA?, , (a) Bacterial artificial chromosome, , (b) Yeast artificial chromosome, , (c) Plasmid, , (d) Cosmid (AIPMT), Commonly used vectors for human genome, sequencing are, , (a) T-DNA (b) BAC and YAC, , (d) T/A cloning vectors., , (APM), , Which of the following is not a characteristic of pBR322, , vector? /, {a) It is the first artificial cloning vector constructed in, , 1977 by Boliver and Rodriguez., , (c) expression vectors, , M., , 35., , 37., , (bh) [tis the most widely used, versatile, nuinipulited veetor,, , It has twa antibiotic resistance genes, te!, amp" Holic resistance genes, ted and, , It does not have restriction site for Sul £, , and easit y, , fe), , «@ (Alittsy, tn order to induce the bacterial uptake of plasmids, the, bacteria are made ‘competent by first treating with, , (a) sodium chloride (b) potassinn chtoride, {c) magnesium chloride (d) calcium chloride, , (AM), Which of the following techniques is most Widely, employed to check the progress of restriction enzyme, digestion?, (a) Agarose pel electrophoresis, (b) Centrifugation, (c) Polyacrylamide gel electrophoresis, (d) PCR (AU), The first recombinant DNA was constructed by linking, an antibiotic resistant gene with the native plasmid of, (a) Escherichia coli, (b) Salmonella typhimurium, (c) Clostridium butylicum, (d) Acetobacter aceti, (e) Bacillus thuringiensis. (Kerala PMT), , Identify the DNA segment which is not a palindromic, sequence,, , (a) 5°GGATCC 3 (b) 5° GAATTC 3", , 3’ GGTACC 5’ ¥ CTTAAG, (c) SGoGcGccoccy (d) 5° CCCGGG 3”, ¥ GGCCGGCG 5° 3 Gascce, (Kamataka CET), EcoR1 is, , (a) used to join two DNA fragments, (b) arestriction enzyme, , (c) the abbreviation for bacterium Escherichia coli, (d) a plasmid, (Kamataka CET), During gene cloning, the enzyme used to join the DNA, with the plasmid vector is, , (a) DNA ligase, , (b) restriction endonuclease, (c) alkaline phosphatase, (d) exonuclease., , (W8 JEE), , 2013, , 40., , DNA fragments generated by the restriction, endonucleases in a chemical reaction can be separated, by, (a), (b), {c), (d), , electrophoresis, , restriction mapping, centrifugation, , polymerase chain reaction,, , (NEET)

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which of the following ts a Palindtromic «, G : equence?, (b) 8" CGAATG-¥, ~COAATG . 8°, ~GACTAC, TACGAC - §°, (AIMS), , ae, , , , ah, , “ae, , ¥-CTTAAG - 5’, , gw hich of the following as a cloning vector?, , (a) DNA of Salmonella typhimurium, , (b> Ti plasmid, , (co) Any DNA containing antibiotic, , (a) Ori minus pBR322 ‘estanse Alles), 43, Foo Ri cleaves the DNA strands to produce, , (a) blunt ends (b) sticky ends, , (c) satellite ends (d) ori replication end., , (Kamataka CET), , 44, Match the entries in column | with those, and choose the correct answer. oh coma Tt, , Column I Column I, , (A) Restriction endo- (p) Kohler and Milstein, nucleases, , (B) Polymerase chain — (q)_ Alec Jeflreys, reaction, , (C) DNA fingerprinting (r) Arber, , (D) Monoclonal (s) Karry Mullis, antibodies, , (a) A- (1). B-(s), C= (q), D-(p), , (b) A-(r), B-(q). C - (8). D-(p), , (c) A-(q). B-(r),C- (8). D-(p), , (dé) A-(q), B-(s), C- (9. D-(q), , 2012, , 48. Which one of the following represents @ palindromic, sequence in DNA?, (a) 3°-GAATTC- 3" (b) 5° - CCAATG- J, 3’- CTTAAG - 5’ 3 -GAATCC-5', (c) 8'- CATTAG-3— (d) 5S - GATACC - 3’, 3'- GATAAC - 5° 3° -CCTAAG - 5, (AIPMT Mains), , (Karnataka CET), , 46. Biolistics (gene-gun) is suitable for, (a) disarming pathogen vectors, (b) transformation of plant cells, (c) constructing recombinant DNA by joining with, vectors :, (4) DNA fingerprinting. (AIPMT Mains), 47. A singe strand of nucleic acid tagged with a radioactive, molecule is called, (a) vector, (c) plasmid, , (b) selectable marker, , (d)_ probe., (AIPMT Prelims), , 48. The given figure isthe diagrammatic representation of the, F. coli vector pit 322. Which one of the given options, correetly identifies its certain companent(s)?, , Feary ites d tit, , , , (a) ori-original restriction enzyme, , (b) rop-reduced osmotic pressure, , {c) Hindlll, EcoRI - selectable markers, (4) amp®, ret" antibiotic resistance genes., , (AIPMT Prelims), , 49. Which one of the following is a case of wrong, matching?, , {a} Somatic Fusion of two diverse cells, , hybridisation, (b) Vector DNA _ Site for rs RNA synthesis, (c) Micropropagation ~ in vitro production of plants, in large numbers, — Unorganised mass of cells, produced in tissue culture, (AIPMT Prelims), , 50. For transformation, micto-particles coated with DNAto, be bombarded with gene gun are made up of, (a) silver or platinum (b) platinum or zinc, (c) silicon or platinum —(d)_ gold or tungsten., (AIPM Prelirns), 51. The restriction enzyme(s) used in recombinant DNA, technology that make staggered cuts im DNA leaving, sticky ends is/are, (a) EcoRI (b) Hindlll, (c) Bamttl (d) all of these. (AMU), , 2, Restriction enzyme Eco RI cuts the DNA between bases, Gand A only when the sequence in DNA ts, {a) GATATC (b) GAATTC, (ec) GATTCC (d) GAACTT. (AMU), , §3. Cohen and Boyer isolated an antibiotic resistance gene,, by cutting out a piece of DNA from a plasmid which, was responsible for conferring antibiotic resistance, 19, the year, (a) 1962 (b) 1965, (c) 1972 (ad) 1982. (Amu), , 54, Inagenctic engineering experiment, restriction enzymes, can be used for, (a) bacterial DNA only, (ce) any DNA fragment, , (d) Callus, , (b) viral DNA only, (d) eukaryotic DNA only., (U8 KCEN

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55. Which one of the following palindromic base sequences, in DNA can be easily cut at about the middle by some, Particular restriction enzyme?, , (a) 5°. CGTTCG -3 (b) 5°-GAATTC - 3’, 3’-CCAAGC - 5’ 3'- CTTAAG - 5°, (©) S'-CTACTG-3" — @)_ $"- CACGTA-3°, , 3'- GTGCAA- 5° 3'- GCATAC - 5, (©) S'-CGAATG - 3°, 3'- CGAATG - 5° (Kerala PMT), , 56. Gel electrophoresis is used for the, , (2) separation and isolation of DNA fragments, , (b) construction of recombinant DNA by joining with, cloning vectors, , (c) culturing the host cells in a medium at a large, scale, , (d) replication of DNA for many times, , (e) cutting of DNA into fragments. {Kerala PMT), , 57. The usual source of restriction endonucleases used in, , gene cloning is, , (a) fungi (b) bacteria, , (c) plants (d) viruses, (WB JEE), 2011, , 58. Given below is a sample of a portion of DNA strand, giving the base sequence on the opposite strands. What, is so special shown in it?, , x GAATTC’ x, x CTTAAG s, (a) Replication completed, (b) Deletion mutation, (c) Start codon at the 5’ end, (d) Palindromic sequence of base pairs, (AIPMT Prelims), , 59. There is a restriction endonuclease called Eco RI, What, does “co” part in it stand for?, (a) Colon (b) Coelom, (c) Coenzyme (d) Coli (AIPMT Prelims), 60. Agarose extracted from sea weeds is used in, (a) spectrophotometry (b) tissue culture, , (ec) PCR (d) gel clectrophoresis,, (AIPMT Prelims), , 61. The thermostable enzymes, ‘Taq’ and ‘Pfu’, isolated, , from thermophilic bacteria are, , (a) RNA polymerases ., , (b) DNA polymerases, , (c) restriction endonucleases, , (d) DNA ligases, (AMU), , 52. The term “molecular scissors” generally refers to, (a) DNA polymerases, (b) RNA polymerases, (c) restriction endonucleases, , (d) DNA ligases. (AMU), , |. Which of the following is correctly matched?, (a) Agrobactertum tumefaciens — Tumour, (b) Thermus aquaticus Bt-gene, , (ec) pBR322 ba reine, , {d) Ligase = Olecular sci, , (e) Hind U ~ Plasmid veceo,, (Keratg Pun), , 64, The term, Southern Blotting’ refers to, transfer of DNA fragments from in vitro, , 2 membrane to electrophoretic gel Cellulose, (b) attachment of probes to DNA fragments, , transfer of DNA fragments from el,, ° gel to nitrocellulose sheet Setrophoretie, , (d) comparison of DNA fragments from two, (Karnataka cen, 65. Plasmids are, (a) extra chromosomal DNA which can self replicate, (b) DNAcarrying genetic sequence, without expressing, it, , (c) integrated within host DNA without Teplicatiog, , ability, (d) none of these. (04g), , 66. Which of the following is not correct statemen, the plasmids ? 7 oo, (a) It is the extra chromosomal DNA in bacteria,, (b) It is nota integral part but inert genetic material,, (c) Host chromosome can be integrated with the, , plasmid,, (d) Transfer of plasmid can be done from cell to cell, without killing the host, (QUE), , 67. Restriction enzymes are used to cut, (a) single stranded RNA (b) double stranded DNA, (c) single stranded DNA (d) double stranded RNA,, , (WB JEE), 68. Biolistic technique is used in, (a) tissue culture process, (b) gene transfer process, {c) hybridisation process, (d) germplasm conservation process. (WB JE}, , 2010, , 69. Which of the following are used in gene cloning?, (a) Nucleosides (b) Lomasomes, (c) Mesosomes ({d) Plasmids, (AIPMT MMaiiis), 70. In genetic engineering, a DNA segment (gene) of, interest, is transferred to the host cell through a vector., Consider the following four agents (i-iv) in this, and select the correct option about which one or more of, these can be used as a vector/vectors., (i) Bacterium (ii) Plasmid, (iii) Plasmodium {iv) Bacteriophage 4