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Dr.Babasaheb Ambedkar Marathwada University, Aurangabad, Dr Rafiq Zakaria Campus,, Maulana Azad College of Arts,Science and Commerce,, Rauza Baugh, Aurangabad,, Department of Biotechnology, ENZYME TECHNOLOGY, UNIT TEST I (20-21), , . Which of the following is an advantage of enzymes over chemical catalysts?, a) High productivity, less material cost, b) High cost of enzyme isolation, c) High cost of enzyme purification, d) Unstable nature of enzymes, . Enzymes discriminate between similar parts of same molecules. This is, termed as, a) Stereospecificity, b) Absolute specificity, Cc) Regiospecificity, d) Group specificity, Enzymes that catalyze the transfer of atom or group between two molecules, is known as, a) oxidoreductases, b) transferases, c) ligases, d) isomerases, . Which of the following belongs to the class of isomerases?, a) Aldolases, b) Oxygenases, c) Epimerases, d) Esterases, . The class of enzymes which contains extensive group of enzymes are, , a) Ligases, , b) Oxidoreductases, , c) Aldolases, , d) Esterases, , . The techniques which adopts the hypertonic and hypotonic condition for cell, lysis is referred to as, , a) EDTA treatment, , b) Ultrasonification, , c) Osmotic shock, , d) Homogenization

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7. The technique in which the cells are subjected to cold temperature of about 15° C to -25° Cis referred to as, a) ultrasonics, b) freezing, c) homogenization, d) osmotic shock, 8. Which of the following methods cannot be used to isolate enzymes from cell, free extract?, a) Use of factors like pH and ionic strength, b) Use of chemicals, c) Homogenization, d) Use of temperature factor, 9. . Which of the following is the best method for isolating enzymes from cell, free extract?, a) pH treatment, b) Temperature treatment, c) Chemical treatment, d) Osmotic shock, 10. The pH at which the net charge on the enzyme molecule is zero is called, , a) pK,, , b) Half-life, , c) Isoelectric point, d) K.,