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+, , , , 1 lotechnology, , , , The development and utilization of biological, forms. Products or Processes for obtainin z, maximum benefits to man and other fi if, life is known as sr forms of, (A) Nanotechnology _, , B ., (C) Zoology (B) Biotechnology, , (D) Botany, , ‘Biotechnology is the aj i ienti, , u g pplication of scientific, and engineering Principles to the Processing of, materials by biological agents to provide goods, and service to the human welfare’ :, The given definition of biot i, , ae jotechnology is, , (A) Indian Council of Agricultural Research, , (B) Genetic Engineering Approval Committee, , (C) Organization for Economic Cooperation, and Development, , (D) Indian Institute of Technology, , Traditional biotechnology was primarily based, on technology using microorganisms., (A) rDNA (B) _ fermentation, (C) cDNA (D) Both (A) and (C), , Which of the following products are NOT, produced using fermentation technology?, , Curd, Ghee, Relaxin, Vinegar, Insulin, Yoghurt,, Cheese, Wine, Erythropoietin, , (A) Insulin, Relaxin, Curd, Cheese, , (B) Curd, Cheese, Vinegar, Erythropoietin, (C) Erythropoietin. Insulin, Vinegar, Curd, (D) Relaxin, Insulin, Erythropoietin, , was developed and then established by, Stanley Cohen and Herbert Boyer in 1973., (A) Fermentation technology, (B) Recombinant DNA technology, , (C) cDNA technology, (D) Transformation process, , e@ 12.2 Principles and, ______ Blotechnology, 6. Modern biotechnology 1s based on two core, , techniques viz., (A) Genetic engineering, (B) Chemical engineering, , Processes of, , , , , , (C) Physical engineering, (D) Both (A) and (B), Tr deals with = maintaining _ sterile, , environment for manufacturing variety of useful, products including vaccines, antibodies,, enzymes, organic acids, vitamins, therapeutics,, etc., , (A) Genetic engineering, , (B) Physical engineering, , (C) Chemical engineering, , (D) Civil engineering, , 8. The manipulation of genetic material towards a, desired end, in a directed and predetermined, way, using in vitro process known as _, (A) chemical engineering, (B) physical engineering, (C) genetic engineering, (D) protein engineering, , 9: Which of the following characterization, techniques are based on molecular weights of, compounds?, iis Gel permeation, ii. Agarose gel electrophoresis, iii. Ion exchange chromatography, iv. Spectroscopy, v. Mass spectrometry, , , , vi. | Osmotic pressure, (A) i, ii, iti, and vi (B) i, ii, iii, iv. Vv, vi, (C) iiandvi (D) iii, iv, v and vi

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10., , 15., , 16., , ==, , Which of the following are the different, methods of electrophoresis?, , (A) Agarose gel electrophoresis, (B) PAGE, (C) SDS PAGE, , (D) All of the above, , Which of the following technique is used for, gene cloning or gene multiplication in vitro?, , (A) Agarose gel electrophoresis, , (B) Gel permeation chromatography, (C) Polymerase Chain Reaction, , (D) SDS PAGE, , The device required for PCR is called, , (A) _ thermal operator, (B) thermal cycler, (C) spectroscopy, , (D) mass spectrometry, , Which of the following are the requirements for, PCR?, , i. DNA containing the desired segment to, be amplified, , ii. | Deoxyribonuclueoside triphosphates, (dNTPs), , iii. | Two primer molecules, iv. Tag polymerase, , v. Mg ions, (A) Onlyi,iiiandv (B) Only ii, iii and v, (C) Only ii and iv (D) i, ii, iii, iv and v, , Which of the following represents the correct, order of PCR?, , (A) Denaturation -— Polymerization —, Annealing of primers, (B) Annealing of primers + Denaturation >, , Polymerization, Polymerization — Annealing of primers, — Denaturation, Denaturation > Annealing of primers >, Polymerization, , (©), @), , Match the column I (PCR steps) and Column II, (Temperature). Select the correct option., , “Column tf Column TF}, <. APCR steps). ...j... ....) (Temperature), |!, Polymerization | P.; 40-60°C, _10-75°C, , ig, :, , , , , , , , , , , , (A) i-S,ii-R,i, (B) i-R,ii-S,ii-P, (C) i-Q ii-R,iii-P, (D) i-P,ii-R, iii-S, , One cycle of PCR takes around =, (A) 3-4 seconds (B) 3-4 hours, {C) 3-4 minutes (D) 30-40 minutes, , , , 17., , 19,, , 20., , 21., , 22., , 23., , 24., , 25., , 26., , Pairing of primers to the ssDNA segment is, , called, (A) (B), (C) (D), , Identify the technique used for purification of, amplified DNA using PCR., , denaturation, , annealing, de-annealing, , polymerisation, , (A) Osmotic pressure, (B) Electrophoresis, (C) Spectroscopy, , (D) Mass spectrometry, , Which of the following are biological tools used, in rDNA technology?, , (A) Enzymes (B), (C) Competent host (D), , Enzymes that cut the phosphodiester bonds of, polynucleotide chains are known as, , Cloning vectors, All of the above, , (A) alkaline phosphatase, (B) nuclease, , (C) DNA ligase, , (D) reverse transcriptase, , Identify the enzyme that cuts nucleotides from, the ends of DNA strands., (B) DNA ligase, , (A) Exonuclease, (C) Tag polymerase (D) Endonuclease, , Which of the following is the CORRECT role of, restriction enzymes in bacteria?, , (A) They help in DNA replication., , (B) They cause destruction of viral DNAs., (C)_ They help in plasmid replication., , (D) They help in bacterial cell division., , Restriction enzymes recognize and cut DNA at, specific sites known as °, , (A) Restriction site (B) Methylated sites, (C) Recognition sites (D) Both(A) and (C), , The restriction enzymes recognize a specific, , , , restriction site which contains, nucleotides., , (A) 12-15 (B) 4-8, (C) 15-30 (D) 20-25, , From the following identify the INCORRECT, pair., , fee Restriction, , , , |, , , , , , , , , , i ii Source, , Misia s crab ON ZY OS sitions ci wise s, LAA) Aled Arthrobacter luteus __, _(B) | EcoRl__|_ Escherichia coli Ry13_, [ (CC) Bam Bacillus thuringiensis H, l, , Hindi | A influenza Rd, , The sequence of nucleotides which can be read, same in forward and reverse direction is known, as, , (A), (B), , , , asymmetrical sequence, supplementary sequence

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|, :, ;, ;, :, , 27., , 28., , 29., , 30., , 31., , 32., , 33., , 36,, , , , (C) _ palindromic sequence, (D) complementary sequence, , , , Vectors are, , t molecules that cai, , eign DN. i wiside the, pret A segment and replicate inside the, (A) RNA (B) protein, (C) DNA (D) lipid, , Which of the following is NOT, plasmid vector?, (A) pBR322, (Cc) M13, , an example of, , (B) puc, (D) Ti plasmid, , Identify the INCORRECT match from the, following., , (A) YAC - Yeast Artificial Chromosome, , (B) MAC - Mammalian Artificial, Chromosome, , (Cc) BAC = Baculovirus Artificial, Chromosome, , (D) Plasmid — pBR322, , From the following identify the plasmid., (A) All, (B) HindIl, (C) EcoRI (D) pBR322, , 12.3 Methodology of rDNA Technology, , The first step in recombinant DNA technology, is, , (A) _ isolation of DNA, (B) insertion of DNA, (C) joining of DNA, , (D) duplication of DNA, , DNA isolated and purified from donor organism, is cleaved with, , (A) _ alkaline phosphatases, (B) _ restriction endonucleases, (C) _ reverse transcriptase, , (D) DNA polymerase, , A DNA fragment with the desired gene is, , known as, (B) chimeric DNA, , (A) foreign DNA, (C) passenger DNA (D) both (A) and (C), , A desired gene can also be obtained directly, from : /, , (A) cDNA library (B)__ t-RNA library, (C) genomic library (D) both (A) and (C), By which of the following process competent, host can take up chimeric DNA? ., , (A) Translation (B) Transcription, (C) Transformation (D) Translocation, , The transfer of rDNA into a bacterial cell is, , assisted by ;, (A) monovalent Zn" (B) divalent Mo"*, (D) divalent Ca, , (C) divalent Co”, , , , 37., , 38., , , , The competent host cell that has taken up, chimeric DNA is known as :, , (A) complementary cell, (B) translated cell, , (C) _ transformed cell, (D) _ transcripted cell, , The desired product produced by transformed, cell is isolated and purified through, , (A) _ upstream processing, , (B) agarose gel electrophoresis, , (C) downstream processing, , (D) mass spectrometry, , 12.4 Applications of Biotechnology, , Match Column I (Health condition) with, , Column II (Recombinant products) and select, the correct option., , , , , , PT Column I | | Column If 4, (Health (Recombinant |, condition)... |. proteins) a8, Cancer, ml Factor 1X, ii. . Urokinase, Tissue, Asthma = plasminogen activator |, iii R. | Interferons,, , Haemophilia B interleukins, _|, , , , , , , , | Q. |, | {, PR, ||, Lt, , , , “Dissolving Ss, _|..._blood clot _ Interleuk’ ee, (A) i-P,ii- S,iii-R,iv—Q, (B) i-R,ii- Q iii-P,iv-S, (C) i-Q ii- S,iii-P,iv-R, (D) i-R,ii- S,iii-P,iv-Q, , 40., , Al., , 42., , 43., , Identify the recombinant protein that is used in, treatment of emphysema., , (A) Factor VII (B) a-1 antitrypsin, (C) Insulin (D) Relaxin, A is a biological preparation that, , provides active acquired immunity against a, certain disease., , (A) antibiotic (B), (C) a-lantitrypsin (D), , The treatment of disease by replacing, altering, or supplementing a gene that is absent or, abnormal and whose absence or abnormality is, responsible for the disease is known as, , vaccine, DNase, , (A) RNA therapy (B), (C) cDNAtherapy (D), , In which of the following ways gene therapy, , can be used?, , i. Replace missing or defective genes, , ii, Deliver genes that speed the destruction, of cancer cells, , iii. By introducing gene in an edible plant, , gene therapy, mRNA therapy, , , , sora

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44,, , 46., , 47., , 48., , 49., , iv. Supply genes that cause cancer cells to, revert back to normal cells, , Vv. Deliver bacterial or viral genes as a form, of vaccination, (A) i, iiand iii (B) ii, ivandv, , (C) iii, iv andy (D) iiiandv, , In which of the following methods genes can be, introduced in sperms, eggs or early embryos?, (A) Bone marrow gene therapy, , (B) Germ line gene therapy, , (C) Somatic gene therapy, , (D) Ex vivo protein therapy, , For which of the following diseases/disorders, somatic gene therapy cannot be performed?, Cancer and rheumatoid arthritis, SCID,, Gaucher's disease, Hypertrichosis, Klinefelter, syndrome, familial hypercholesterolemia,, Tumer syndrome, phenylketonuria, cystic, fibrosis, Duchenne muscular dystrophy, Down, syndrome, emphysema, thalassemia, (A) Familial _hypercholesterolemia,, syndrome, phenylketonuria, SCID, (B) Tumer syndrome, Klinefelter syndrome,, Down syndrome, Hypertrichosis, (C) Duchenne muscular dystrophy, Cancer, and rheumatoid arthritis, Gaucher’s, disease, Emphysema, Gaucher’s disease, _ Hypertrichosis,, Klinefelter syndrome, Familial, hypercholesterolemia, , Down, , (D), , The living organisms whose genetic material, has been artificially manipulated in a laboratory, through genetic engineering are known as, , (A) Chemically Modified Organisms (CMO), (B) Genetically Modified Organisms (GMO), (C) Synthetically Modified Organisms (SMO), (D) Both (A) and (B), , Which of the following is the first transgenic, plant?, , (A) Tomato (B), (C) Tobacco (D), , Wheat, Rice, , Transgenic plants are being looked up as, for production of novel drugs like, , interferons, edible vaccines, antibodies, amino, , acids, immunotherapeutic drugs, etc., , (A) _ bioreactors (B) _ bioregulators, , (C) biotransformers (D) _ bioterminators, , The cry gene present in produces a, protein that forms crystalline inclusions in, bacterial spores., (A) A. tumefaciens, (C) E.coli, , (B) B. thuringiensis, (D) _ S. pneumoniae, , , , 50., , 52., , 53., , 54., , 55., , 56., , 57., , Pore, , , , , Insect resistant plants contain either a gene from, B. thuringiensis or the, , (A) Rhizopus arrhizus, , (B) cowpea trypsin inhibitor gene, , (C) Acetobacter aceti, , (D) Aspergillus niger, , Bt toxin activity has been observed: against, many species of insects within the orders of, (A) Lepidoptera (B), (C) Coleoptera (D), , Golden rice - a transgenic food crop used to, , Diptera, All of the above, , reduce deficiency disease. ;, (A) vitamin D (B) vitamin €, (C) vitamin By (D) vitamin A, , Match the column I (Substance) with column I, (Transgenic Crop) and select the correct option., , , , , , , , , , , , , , , , sae _Column I : _ Column IL, __Flavonoids Mecha RICE |, b. __Canola, : = _.Tomato, L _d._| Sugar beet, (A) -a,iv—c, (B) i-c,ii-d,iii-a,iv—b, , (C) i-a,ii-d,iiic,iv—b, (D) i-c,ii-d, iii-b, iv-a, , The improved oil content and oil quality of oil, crops like soybean, oil palm, rapeseed and, sunflower, have been achieved by transfer of, , (A) cry gene (B), (C) Arabidopsis gene (D), , , , a-1 antitrypsin, Phytoene gene, , The genes for isolated from soybean, and Phaseolus have been transferred to rice to, overcome iron deficiency., , (A) B-carotene (B), (C) y—tocopherol (D), , Assertion (A): Flavr savr tomato has longer, , shelf life., , Reason (R): In Flavr savr variety of tomato, the, , enzyme polygalacturonase is produced in less, , amounts., , (A) Both A and R are true and R is the correct, explanation of A., , (B) Both A and R are true and R is not the, correct explanation of A., , (C) Ais true and R is false., , (D) Both A and R are false., , Biopharmaceuticals are proteins, hormones,, antibodies, vaccines or enzymes isolated from, , ferritin protein, flavonoids, , (A) dwarf plants (B), (C) herbicide plants (D), , transgenic plants, A. tumefaciens

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58., , 59., , 61., , 62., , 63., , , , , , , , , , Read the following statements, , proteins that are being produce, , crop plants. Select the Correct 0, , i. Human testosterone hort, by gene inserted into the, of tobacco plants,, , ii. In transgenic plants. H i, _ S, umanized, i can be produced against HIV., ‘espiratory syncytial virus (R: :, simplex virus (HSV) (RSV), Herpes, Protein antigens to be used in vaccines for, e.g. Patient-specific, (a cancer) vaccines,, (A) _ iiand iii are correct,, (B) i and ii are correct,, (C) i and iii are correct, (D) i, iiand iii are correct,, , with respect to, d by transgenic, ption,, , mone is produced, chloroplast DNA, , iii., , antilymphoma, , B-cell lymphomas are clones of, , i : cells, expressing on their surface a u, , nique antibody, , molecule., , (A) normal B (B) malignant B, , (C) normal RBC (D) malignant T, Which of the following is a antimalarial drug?, (A) Tetracycline (B) Ampicillin, , (C) Artemisinin (D) Chloromycetin, Which of the following statement _ is, INCORRECT with respect to transgenic, animals?, , (A) Transgenic animals are used as producers, , of specific proteins., (B) Transgenic animals are specially created, for use in xenografting., (C) Transgenic animals cannot be used in, medical research field., (D) Transgenic animals, physiology and anatomy., , have improved, , The oncogenes myc and ras were analysed to, , find out if they lead to in mice, transformed with these genes., (A) | skin cancer (B) lung cancer, , (C) _ breast cancer (D) prostate cancer, , To increase wool production and to improve the, quality of wool, the bacterial genes _ and, concerned with biosynthesis of, cysteine “anino acids involved in formation of, keratin protein found in wool are cloned and, introduced in sheep., (A) cys E,cysM (B), (CC) cys F,osG (D), , Which of the following properties are observed, in transgenic fish?, , cys F, cys M, lac y, laca, , , , 65., , @ vs, , 66., , 67., , 68., , 69., , 70., , 71., , Atlantic salmon, catfish, goldfish, Tilapia,, zebra-fish, common carp, rainbow trout, etc. are, the commercially important fishes., , From the following identify the components that, are used for improving fish quality., , (A) Growth hormone, , (B) Chicken crystalline protein, , (C) E.coli hygromycin resistance gene, , (D) All of the above, , Bioethics, Biopiracy and Biopatent, , Biopiracy is related to which of the following?, (A) Bioresearches, , (B) Traditional knowledge, , (C) Biomolecules and genes, , (D) All of these, , The right granted by government to an inventor, to prevent other from commercially using his, invention 1s, , (A) patent (B), (C) _ bioethic, , biopiracy, (D) biowar, , Biopatent can be awarded for, , (A) strains of micro-organisms, cell lines,, , genetically modified organisms, , (B) DNA sequences and proteins encoded, , (C) biotechnological procedures, processes,, products and product applications, , (D) all of these, , A patent consists of, (A) _ grant (agreement with the inventor), (B) _ specification (subject matter of invention), , (C) claims (scope of invention to be, protected), (D) all of the above, , In 1997, the granted a patent to a, Texas based American company Rice Tec Inc., for “Basmati rice line and grains”., , (A) US Piracy and Trademark Office, (USPTO), , (B) US Patent and Trade work Office, (USPTO), , (C) US Patent and Trademark Office, (USPTO), , (D) US Piracy and Trade Approval Office, (USPTAO), , Among the following, which group of products, were awarded with false patents?, , (A) Basmati rice, Turmeric, Neem, , Id tolerance, S ferred oe (B) Golden Wheat, Red rice, Aloe vera, (C) Cry protein resistance (C) Long threaded jute, Cotton, Golden rice, (D) Both (A) and (B) (D) Tomato, Hibiscus, Rose