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Nucellus Culture, , Nucellus is the main bulk of ovule in seeds. Itis diploid, and parenchymatous. A portion of nucellus is excised from, fertilized ovule and cultured into embryoid. The embryoid is, then grown into'a plantlet. Such plants are free from viruses., Nucellus culture is practiced in citrus varieties., , The simplified procedure for nucellus culture is given, below:, , 1. Immature green fruits of citrus are harvested., , 2. The fruits are washed with soap and cleaned with, distilled water. :, , 3. The fruit is cut with a sterile knife and the ovaries, are collected., , 4. The ovaries are surface-sterilized with 2.4% sodium, chloride solution for about 15-30 minutes., , 5. The ovules are taken from the surface-sterilized, ovaries with the help of the sterile knife. a, , 6. The nucellus is taken from the surface-sterilized., seeds or ovules by cutting them longitudinally with the help, of forceps and scalpels under a dissection microscope., , 7. The entire nucellus tissues are inoculted in a flask, containing M.S. medium supplemented with malt extract, (100g/l) and White’s vitamins. © : a, , 8. The.culture flask is then incubated at 25-29°C, wi, 100 lux illumination for about 2-6 weeks. During incubation,, the nucellus tissues undergo differentiation and develop into, , embryos.