Page 1 : Exhaustive methylation: The process of, alkylation with methyl groups until all, possible methylations have been achieved., , Ve LQe5.5 2, , In the Hofmann einen reaction, sequence, exhaustive methylation (shown, in red) converts an amine to an, alkylammonium salt prior to E2, elimination., , oe :, Z Sat ie, \ KO; HA b, | hate Si ., Re N 21, N |, ae, , Exhaustive methylation has been used in, natural product structural analysis. For, example, if one mole of an unknown, alkaloid (represented here by nicotine), reacts with two moles of methyl iodide, we, can conclude the alkaloid contains two site, for methylation.